海洋硅藻高效固碳的酶促协同机制研究

海洋浮游硅藻是海洋初级生产力的重要组成部分，其高效的固碳能力与核酮糖-1，5-二磷酸羧化酶/加氧酶（Rubisco）和碳酸酐酶的酶促作用密切相关。本项目选择具有不同固碳途经的海洋浮游硅藻三角褐指藻（C3途径）和威氏海链藻（C4途径）作为实验材料，通过Rubisco和碳酸酐酶活性抑制实验，探讨两者活性变化的协同性与光合固碳效率的关系；利用免疫荧光技术研究Rubisco与碳酸酐酶的共定位（Co-localization）关系及其与光合固碳效率的相关性；通过环境因子（光和CO2等）的调控作用研究Rubisco和碳酸酐酶的环境协调性，同时比较不同固碳途径硅藻类群Rubisco与碳酸酐酶协同性的差异。通过以上研究阐明在海洋硅藻光合固碳过程中Rubisco和碳酸酐酶的协同机制，揭示Rubisco和碳酸酐酶的协同作用在海洋浮游硅藻碳代谢中的重要意义。

Marine planktonic diatom is an important part of marine primary productivity, and which capacity of efficient carbon fixation are closely related to the enzymetic catalysis of carbonic anhydrase (CA) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). In this project, Phaeodactylum tricornutum with C3 pathway and Thalassiosira weissflogii with C4 pathway are selected as experimental materials to investigate the relationship between the efficiency of carbon fixation and the synergy of Rubisco and CA activity. The co-localization between Rubisco and carbonic anhydrase is also studied by immunofluorescence technique. The environmental synergy bewteen Rubisco and CA is also investigated by the regulation of light and CO2. At the same time, the differences bewteen the synergies of CA and Rubisco in diatoms with the different pathway of carbon fixation are compared. The above study will state the synergistic mechanisms bewteen CA and Rubisco in the photosynthetic carbon fixation, and reveal the role of CA and Rubisco in the carbon metabolism of marine planktonic diatom.