海洋硅藻CO2浓缩机制与Rubisco对大气CO2浓度升高和海洋升温的响应及其机制研究

大气CO2浓度升高和海洋升温是全球关注的重大环境问题。海洋硅藻光合作用如何响应和适应环境变化与其CO2浓缩机制（CCM）和核酮糖-1,5-二磷酸羧化/加氧酶（Rubisco）密切相关，但目前CCM和Rubisco对大气CO2浓度升高和升温的进化性响应及其机制尚不明晰。本项目以三角褐指藻为实验材料，设置不同CO2浓度（400和1000ppm）和温度(15-25℃)实验，通过短期或长期培养，利用膜进样质谱等技术研究CCM效率的变化；从生化和转录水平厘清大气CO2浓度升高和升温对碳酸酐酶和Rubisco的活性、蛋白量和转录丰度的短期和长期效应，探讨CCM、碳酸酐酶和Rubisco的协同性。通过以上研究阐明海洋硅藻CCM和Rubisco对大气CO2浓度升高和升温的响应机制，揭示CCM和Rubisco在光合作用碳获取和固定中的协同策略，为预测和评估未来全球气候变化对海洋初级生产力的影响提供科学依据。

The increase of atmospheric CO2 concentration and marine warming has raised global concern. How the photosynthesis of marine diatoms responds to and adapts to this change is closely related to CO2 concentrating mechanism (CCM) and ribulose-1,5-diphosphate carboxylation/oxygenase (Rubisco). However, the evolutionary response to elevated atmospheric CO2 and marine warming on CCM and Rubisco are yet unclear. In this project, Phaeodactylum tricornutum was cultured in the different CO2 concentrations (400 and 1000 ppm) and temperature (15-25 ° C) for short or long-term to investigate the changes of CCM efficiency using membrane injection mass spectrometry. We also study the short and long-term effects of atmospheric CO2 concentration rise and marine warming on the activity, protein amount, and transcriptional abundance of carbonic anhydrase and Rubisco from the biochemical and transcriptional levels, and explore the synergy among CCM, carbonic anhydrase and Rubisco. From the above research, the response of CCM and Rubisco in marine diatoms to the increase of atmospheric CO2 concentration and marine warming was clarified, and the synergetic strategy of CCM and Rubisco in photosynthetic carbon acquisition and fixation was also revealed, which provide a scientific evidence to predict and evaluate the impact of global climate change on the marine primary productivity in future.