寡糖对花椒干腐病的影响及作用机制研究

基于寡糖在植物病害系统中的调控作用，本研究以花椒干腐病为研究体系，分别制备花椒（Zanthoxylum bungeanum）和干腐病菌（Fusarium lateritium）总寡糖并研究其对花椒干腐病的影响。通过研究各总寡糖对花椒干腐病菌的室内抑菌活性和对干腐病在花椒枝干上的发展情况，确定对花椒干腐病具有较好控制效果的总寡糖，并探讨其作用机制。对活性总寡糖进行分离纯化以获得寡糖单体，并且对寡糖单体的活性进行验证和构效关系的分析，进一步对寡糖单体的施用条件进行优化，建立防治指标与影响因素之间的二次多项式模型，为利用寡糖防治花椒干腐病提供依据。

In view of the regulating function of oligosaccharide in plant disease system, the biological activities of total oligosaccharides prepared from Zanthoxylum bungeanum and Fusarium lateritium are researched in the dry rot system of Z. bungeanum. By investigating the effects of the total oligosaccharides on the inhibitory activity of F. lateritium in vitro and the development of dry rot on the branches of Z. bungeanum in vivo respectively, the total oligosaccharide possessing excellent activities is screened out and its function mechanism is also discussed. And then the chosen total oligosaccharide would be subjected to isolating and purifying to obtain oligosaccharide monomer. After that, the biological activities of oligosaccharide monomer would be confirmed and the relationship between its structure and activity would also be analyzed. Furthermore, the applying conditions of oligosaccharide monomer are also optimized. A quadratic polynomial model would be established between the detecting indicators and influencing factors, which would guide the use of oligosaccharide for controlling dry rot of Z. bungeanum.