植物用生物钟调控生理活动与外界时空环境同步而增强适应和竞争能力。生物钟环境变化记忆和预测功能近来成为时间生物学的前沿和热点问题。海陆潮间带红树林植物生理活动既受昼夜有规律更替的环境因子的影响，也受节律性动态潮汐的影响，而这两种影响又常交织在一起。我们初步研究发现，与已有报道相比，红树植物光合碳同化和气孔开闭运动生理节律有别于内陆植物，也不同于(海)水生植物。在此基础上，项目以热带雨林山红树为对照，设置现场测定和温室栽培模拟实验，用数理统计和模型拟合方法，弄清潮间带红树植物生物钟对日节律和潮汐节律的记忆、预测能力及对光合贡献程度。为揭示日节律和潮汐节律通过生物钟调控光合作用的途径、方式和机制，项目也拟监测根、叶微管组织信号物质动态和核心钟基因及调节因子表达动态。项目实施为红树林保育恢复及可持续发展提供直接理论指导，为植物生物钟研究提供新资料、新认识，也有助于记忆生态、预测生态新兴学科的繁荣。

The circadian clock increase plants’ fitness and competitiveness by regulating physiological responses to the temporal and spatial changes synchronously. The memorizing and predicting the variations in the environment by circadian clock have gradually been in a hot research in Chronobiology. The physiological activities of mangrove plants grown in the intertidal zone are affected not only by the variation of the environment factors but also by the dynamic fluctuation of the tides, and in most of the time, by both. Our previous study found that the photosynthetic rhythm and stomatal activity rhythm in mangroves are different from both terrestrial plants and (sea) aquatic plant. On this basis, the project aims to study the memory and anticipation of the variations in environment factors and tides by circadian clock and subsequent increase photosynthesis in mangrove plants. Pellacalyx yunnanensis grown in the tropical rain forest will be used as the contrast, several Rhizophoraceae plants grown in the intertidal zone will be selected, and the photosynthetic rhythm in them will be tested. Then, the relationship among the variation of environment factor, the tides and the photosynthetic rhythm will be analyzed based on statistical method and numerical model fitting method, and the contribution of circadian regulation to the increases in photosynthesis will be evaluated. Moreover, to discover the signals input to the circadian clock and the circadian regulations on photosynthesis mechanism, the dynamic changes of the signal substances such as ABA and ROS in the vascular bundles of stem, and the expression of the key clock gene and regulatory factor gene will be analyzed in both roots and leaves. The project implementation will provide theoretical guidance to the conservation and sustainable development of mangrove plants and give new useful data for plant circadian study. Besides, the implementation of the project will contribute to the up and coming field of ecological memory and anticipation.