釉基质蛋白(EMPs)联合牙周膜细胞(PDLCs)用于牙周组织工程技术可以修复牙周缺损促进牙周再生，而局部微环境对参与组织再生的细胞生物学特性的影响至关重要，牙周炎造成的深牙周袋内形成低氧微环境，我们前期研究发现，低氧可诱导人PDLCs中低氧诱导因子1α (HIF-1α) 蛋白表达，但低氧下EMPs对PDLCs的作用及其调控机制研究尚未见报道。本研究将系统分析低氧下EMPs作用PDLCs后HIF-1α表达情况，及其对PDLCs增殖、凋亡和成骨分化的影响；研究PI3K/AKT信号通路在低氧下EMPs作用PDLCs中的调控机制。以期进一步阐明在不同环境条件下EMPs促进牙周再生的机制，有助于寻找促进牙周再生的新靶点，这将为深入研究釉基质蛋白的作用机制和该组织工程技术的临床应用提供理论依据。

Combination of enamel matrix proteins (EMPs) and periodontal ligament cells (PDLCs) could promote periodontal regeneration in repairing periodontal defects. Physiological microenvironments play a crucial role in maintenance of stem cell properties during tissue regeneration. Hypoxic condition was present in the deep pocket resulted from periodontitis. In our previous study, hypoxia induced the expression of HIF-1α (hypoxia-inducible factor-1α) protein. However, it is unclear on the mechanism of the effect of EMPs on PDLCs under hypoxia. This study investigates the expression of HIF-1α in PDLCs treated with EMPs, and the effects of HIF-1α on proliferation , apoptosis and osteogenic differentiation of PDLCs. Then, it will be studied about the regulatory mechanisms of PI3K/AKT pathways in PDLCs treated with EMPs under hypoxia. The aims are to elucidate the regulation mechanisms of EMPs promoting regeneration of periodontal tissues under different conditions, to explore the novel target of regeneration of periodontal tissues, and to provide the theoretical basis for further study of EMPs and clinial appilication of periodontal tissue engineering.