水稻锌指蛋白转录因子HST在花期高温胁迫应答中的功能与调控研究

高温胁迫已成为作物生产的威胁,水稻耐高温机制的阐明和耐高温相关基因的克隆、鉴定及功能解析是水稻耐高温育种的重要基础。本研究以前期获得的一份抗高温能力明显增强的水稻中花11突变体（hst，heat stress tolerance）为材料，经图位克隆与测序发现该突变体中的目的基因发生了移码突变，且为锌指蛋白转录因子，在花期高温胁迫中起负调控作用。为此，本项目采用转基因、ChIP-seq、RNA-Seq等技术和解剖学手段，在花期高温（40℃）胁迫下，研究该锌指蛋白转录因子HST在水稻花期的时空表达模式，寻找HST下游的直接调控靶基因,分析花粉活力、花药结构与结实率、产量等农艺性状及其生理生化（激素、氧化胁迫）指标，探明锌指蛋白转录因子HST在水稻花期高温胁迫响应中的功能与调控网络，为阐明水稻高温热害的分子机理提供科学依据，为作物耐高温分子育种提供理论指导。

Heat stress has become a threat to crop production. It is the important base on the rice heat tolerant breeding to illuminate the mechanism of rice heat stress tolerance, and to clone, identify the genes related to the heat stress tolerance. A heat-resistant mutant of the rice ZH11（hst，heat stress telorance）acquired before was utilized to clone the related genes. With map-based cloning and sequencing, it was found that 4 basic groups were inserted into the zinc finger transcription factor HST, which resulted in the frameshift mutation. According to the above evidences, it can be indicated that this transcription factor was a negative regulator of rice heat stress tolerance. Some technologies and anatomy methods, such as the transgenosis, ChIP-seq, RNA-Seq and so on, will be used to study the spatial and temporal expression pattern of HST, to exploit the downstream target genes of HST at flowering under the condition of 40℃. Meanwhile, the pollen viability, the developmental structure of the anther, the physiological and biochemical indexes and the agronomic traits including the setting rate, the production and so on will be studied, too. All of these will verify the functions and the regulatory network of HST taking part in at the rice flowering under heat stress, illuminate the molecular mechanism of the heat stress in rice and provide theoretical basis of molecular breeding under heat stress.