湿地是温室气体甲烷（CH4）和氧化亚氮（N2O）的重要排放源，其温室气体产生途径主要为水中污染物的微生物分解过程。甲烷厌氧氧化（AOM）在湿地甲烷减排中起到重要的作用，尤其是反硝化型AOM成为近期国际研究的热点。本课题基于对反硝化路径中各中间产物的反应势垒和化学反应热力学分析，提出N2O可作为AOM电子受体的假设，并在此基础上应用同位素示踪法开展N2O依赖型的AOM新路径的研究，结合微生物纯培养、分子遗传学及蛋白质分离纯化技术，明确该路径的微生物学机制，掌握在不同环境中起作用的微生物类群；通过在人工湿地污水处理系统中优化运行工艺参数和植物配置，改善微生物群落结构和功能，构建强化N2O依赖型的AOM人工湿地系统，在有效去除水中污染物的同时，实现CH4和N2O两种温室气体的交互消减。本研究的开展将对控制温室气体排放及扩展对全球碳氮循环的认识具有重要的科学意义。

Wetlands are the main source of methane (CH4) and nitrous oxide (N2O) emissions, which come mostly from microbial decomposition of contaminants. Anaerobic oxidation of methane (AOM) plays an important role in methane mitigation, especially the denitrification-dependent AOM. This project proposes the feasibility of a new path of N2O-dependent AOM based on the transfer barrier and thermodynamics of all intermediates during denitrification. The mechanisms of N2O-dependent AOM will be confirmed by isotope tracer technique. The microbiological mechanisms of N2O-drived AOM under different environment conditions will be investigated by combining the technologies of microbial pure culture, molecular genetics, and separation and purification of protein. To further achieve the mutual reduction of CH4 and N2O emissions, the microbial community in the constructed wetland sewage treatment system will be improved by optimizing influent characteristics, operation parameters and plant arrangement. The results will be vitally important for controlling greenhouse gas emissions and global carbon and nitrogen cycles.