目前针对乳腺癌的胸壁转移或术后胸壁复发的治疗仍然是一大挑战，而成像引导的近红外二区光热治疗对肿瘤的杀伤强且对周围器官损伤小。因而，如何提高光热试剂对乳腺癌胸壁转移或复发部位的靶向性和光热治疗的疗效具有一定的研究价值。申请人前期研究发现硫化铜纳米颗粒具有良好的生物相容性和近红外二区的光热转换能力，且在X射线照射下可促进细胞对其的摄取。基于此，本项目设想以乳腺癌细胞膜为模板原位生长硫化铜纳米颗粒（M-CuS-PEG）来获取对肿瘤的同源靶向和99mTc标记的能力。利用99mTc衰变产生140keV的γ射线，进一步研究和探索该射线是否可以促进细胞对硫化铜的摄取，进而增强其后续的光热治疗疗效并阐明其作用机制。因此，99mTc标记的M-CuS-PEG可实现对乳腺癌胸壁转移或复发部位的核医学成像和诊断性核素增强的光热治疗，为临床上乳腺癌胸壁转移或复发的治疗提供了一个切实可行的参考方案。

Currently, the treatment of breast cancer with chest wall metastasis or recurrence is still challenge. Image-guided photothermal therapy in the near-infrared II region (NIR-II) can exert effective anti-tumor effect and induce little damage to the normal organs. However, it is still difficult to improve the tumor targeting ability of thermal agents to the tumor in chest wall and enhance the therapeutic efficiency of photothermal therapy. In our previous studies, we found that copper sulfide nanoparticles with excellent biocompatibility and photothermal conversion efficiency in NIR-II showed enhanced cellular uptake under X-ray exposure. Therefore, we hypothesize to in situ grow copper sulfide on the breast cancer cell membrane for obtaining nanoprobe with homologous targeting ability and 99mTc labeling ability. Moreover, considering the γ-ray produced by 99mTc, the effect of 99mTc on the endocytosis of copper sulfide and its mechanism will be explored. These biomimetic nanoparticles labeled with 99mTc can be used for Single-Photon Emission Computed Tomography （SPECT） imaging-guided and 99mTc-enhanced photothermal therapy of breast cancer in chest wall. This project provides a reference scheme for the clinical treatment of metastatic or recurrent breast cancer in chest wall.