微RNA介导的长链非编码RNA的调控网络和功能研究

长链非编码RNA（lncRNA）、微RNA(miRNA)和竞争性内源RNA (ceRNA)在细胞功能和命运决定中起关键作用，并影响着肿瘤的发生发展。然而，miRNA-lncRNA和miRNA-ceRNA之间互作的潜在规律及功能还有待阐明。我们前期研究开发了starBase和ChIPBase两大功能分析平台实现对miRNA功能靶标组和lncRNA转录调控网络的研究。在此基础上，本项目拟通过整合CLIP-Seq和转录组数据，开发新的算法和平台在转录组水平系统地鉴定miRNA-lncRNA及miRNA- ceRNA互作网络；进一步结合功能基因组的数据，开发基于ceRNA调控网络预测lncRNA功能的新方法，在神经胶质瘤和结肠癌等高通量癌症基因组数据中鉴定癌症特异的lncRNA及其ceRNA 功能网络，探讨它们在肿瘤发生发展过程中的作用，拓展我们对lncRNA功能及ceRNA调控网络的认识。

Long non-coding RNAs(lncRNAs), microRNAs(miRNAs) and competing endogenous RNAs(ceRNAs) play important roles in deciding cellular function and fate, and also affect the tumorigenesis.However,the potential rules and function of miRNA-lncRNA and miRNA-ceRNA interactions in cell remain to be elucidated.In the previous studies, we have developed starBase and ChIPBase platforms to decode the miRNA targetome and the transcriptional regulations of lncRNAs and miRNAs.In the present project, we will develop new algorithms and analysis platforms based on integration of CLIP-Seq and transcriptomic data to systematically identify the miRNA-lncRNA and miRNA-ceRNA interactome.Furthermore, by combinding functional genomic data, we will develop new methods to predict lncRNA function from the ceRNA regulatory networks involving lncRNAs. Finally, we will apply these methods to the high-throughput genomic data of glioma and colorectal cancer to identify the cancer-specific lncRNAs and ceRNA networks and explore their potential roles in tumorigenesis. Altogether, this study will greatly expand our understanding on the lncRNA function and mechanism of coordinated regulation of ceRNAs.