hsa_circ_001634/miR-194/Wnt轴参与胃癌发生发展的机制研究

预实验中我们发现miR-194在胃癌中促进细胞的生长和迁移，是一个致癌miR。根据生物信息学分析及预实验结果，提出了一个新的miR-194调控机制：即miR-194在hsa_circ_001634调控下，通过SUFU等靶基因激活Wnt信号通路促进胃癌发生和发展。本项目拟利用各种转染、荧光素酶报告系统、CHIP、共聚焦免疫荧光、体内外生物学功能等实验验证这一机制。最终探明（1）hsa_circ_001634和miR-194 在胃细胞中的生物学功能，hsa_circ_001634是miR-194的上游调控因子。（2）hsa_circ_001634/miR-194/Wnt轴信号通路的作用机制。（3）SUFU、AXIN2等是miR-194的靶基因。旨在阐明hsa_circ_001634/miR-194/Wnt轴信号通路在胃癌演进中的作用机制，为临床胃癌诊治提供新的分子标志物和基因靶点。

Based on our preliminary data, miR-194 was identified as an oncogenic miR in gastric cancer (GC). It promoted cell growth and migration in GC cells. According to the bioinformatic analysis and preliminary results, we propose a new regulatory mechanism of miR-194: miR-194, regulated by hsa_circ_001634, contributes to the GC tumorigenesis by activating Wnt signaling pathway via targeting SUFU. We intend to elucidate the mechanism by using different transfection combination, interference, luciferase reporter system, CHIP, confocal immunofluorescence and biological function tests. (1) We would like to explore the biological function of hsa_circ_001634 and miR-194 in gastric cells and determine if hsa_circ_001634 is an upstream regulatory factor for miR-194. (2)We will characterize the hsa_circ_001634/miR-194/Wnt axis signaling pathway in GC tumorigenesis. (3) We would confirm SUFU, AXIN2 are the target genes of miR-194. The project will ultimately reveal a new regulatory mechanism of miR-194 and clarify the function of hsa_circ_001634/miR-194/Wnt axis signaling pathway in GC tumorigenesis. It will provide novel circRNA, miR molecular markers and gene targets for clinical diagnosis and treatment of GC.