鉴于淀粉回生影响因素复杂性和传统研究手段的局限性，回生共性理论长期以来拘泥于高分子淀粉链的有序重排现象，缺乏不同回生阶段关联性解析的实质性突破。特别是短期回生阶段直链淀粉/支链淀粉链内链间晶核是如何影响支链淀粉主导的长期回生问题仍不明确，这也是调控回生开发高品质米面制品所面临的瓶颈。基于此，本项目提出在大米淀粉糊化重结晶过程中，添加短期回生阶段形成的模拟晶种，简化复杂的回生研究体系，诱导淀粉长期回生重结晶过程；建立并运用AFM轻敲模式下的晶粒成像分析、WXRD Jade 6.5晶胞参数解析、荧光客体分子介入荧光光谱的晶体密度测试等淀粉晶态结构解析的先进手段，考察模拟晶种晶态结构特征，明确其作为淀粉异相成核启动子的临界尺寸，从淀粉晶核成核模式、片晶层状结构转化等角度，明确淀粉不同回生阶段关联性，揭示基于模拟晶种作为异相成核启动子的回生共性机制，为高品质米面制品开发提供重要理论基础。

Because of complexity of factors which affect retrogradation and limited studying methods, common theory of retrogradation is not fully understood and verified. Especially, the relationship between amylose or amylopectin crystals- induced short-term retrogradation and amylopectin-induced long-term retrogradation is still unclear, which restricts the development of preparing slowly digestible starch with trophic function by retrogradation and reducing retrogradation for rice & flour products with high quality. As a result, this project simplifies the retrogradation system by using the simulated seed crystal of amylose or amylopectin as the real crystal and accurately controlling the addition of crystal. Furthermore, advanced techniques, including Atomic Force Microscopy with imaging system, Wide X-ray Diffraction with Jade 6.5, and Fluorescence Spectroscopy with fluorescent guest, will be developed and used for analysing the crystalline structure of starch. Based on these studies, we firstly try to find out the critical size of the simulated seed crystal which can act as crystal nucleus to accelerate retrogradation in heterogeneous phase. Secondly, we plan to propose the mechanism for the acceleration and illustrate the relationship between short-term retrogradation and long-term retrogradation from the mode of nucleus growth and transformation of crystal structures. This relationship will help to understand the common mechanism of retrogradation induced by the simulated seed crystal, providing scientific basis for preparation of starchy food products with high quality.