趋磁螺菌MSR-1能从外界环境吸收大量铁，其5个铁吸收调控蛋白对生物矿化具有重要的调控作用，这在非磁细菌中十分罕见。铁响应调控蛋白IrrB作为其中一员，已证明其参与铁氧平衡、抗氧化胁迫和辅助磁小体合成的功能，但其调控机制及与其他成员的调控关系尚不明确。因此，本研究围绕IrrB的表达特点和调控机制，利用转录组测序技术，从不同铁条件的横向水平、磁小体合成不同阶段的纵向水平，分别分析供试菌株MSR-1和irrB缺失突变株的差异基因相关信息，明确MSR-1 IrrB调控的关键基因及代谢途径。借助凝胶迁移和染色质免疫共沉淀等方法，在体外和体内精确验证转录组结果，构建IrrB的全局调控网络，阐明其与MSR-1 Fur的调控关系及与非磁细菌Irr的调控差异，为深入探讨细胞内生物矿化机制、推动纳米磁小体的广泛应用奠定基础。

Magnetospirillum gryphiswaldense MSR-1 can absorb a large amount of iron from the external environment, and its five iron uptake regulator proteins play an important role in biomineralization, which are very rare in non-magnetic bacteria. As one of them, iron response regulator IrrB has been proved to be involved in the functions of iron/oxygen balance, oxidative stress tolerance and magnetosomes synthesis. However, its regulatory mechanism and the regulatory relationship with other Fur members are not clear. In this study, we will use RNA-sequencing to analyze the differential expressed genes and related metabolic pathways under various stages of growth and magnetosome formation in wild type strain WT and irrB deletion mutant (ΔirrB). Based on the accurate verification of transcriptome results, the global regulatory network of IrrB will be constructed with the help of bioinformatics tools. The regulatory network of IrrB helps to elucidate its regulatory relationship with MSR-1 Fur and non-magnetic bacteria Irr, which lay a foundation for further study of biomineralization mechanism and promotion of the wide application of nano-magnetic bodies.