目前，除了生物修复，堆肥被认为是可以增强废弃物处理、土壤有机质含量和土壤肥力的最有效的方法之一。本项目拟以甘肃陇东油田所产的含油土壤为研究对象，基于同位素标记法，采用微生物强化堆肥，通过研究不同微生物在堆肥中对TPH底物的降解性能、演化特征，探讨细菌真菌的协同降解机理；然后，利用标记底物在联合降解和单一降解体系中的降解演化差异及新形成的组分（含有重同位素）差异，并跟踪微生物在降解演化中的群落动态变化，揭示外源与内源微生物在降解中的相互作用；最后，通过调控堆肥体系中标记有机质的含量以及其分散状态，研究其对微生物菌群的分布和数量影响，从微观层次揭示有机质对微生物降解TPH的影响规律，以期为实现含油污泥的无害化处理和农用废弃物的综合利用提供重要的科学依据。

At present, composting is considered as one of the most effective ways to enhance waste disposal, soil organic matter content and soil fertility, except for bioremediation. This project intends to use oil-contaminated soil produced by Gansu Longdong Oilfield as the research object, based on isotope labeling, microbial enhanced composting is used to study the degradability and evolution characteristics of different microorganisms in composting to TPH substrate and to explore the synergistic degradation mechanism of bacterial and fungi; Then, using the differences of degradation and evolution of labeled substrates in combined degradation and single degradation systems and the newly formed components (including heavy isotopes), and follow the community dynamics of microbial evolution in degradation to reveal the interaction of exogenous and endogenous microorganisms in degradation; Finally, by regulating the content of labeled organic matter in composting system and its dispersion status, study the influence on the distribution and quantity of microbial flora, and reveal the influence of organic matter on the degradation of TPH by micro-level, in order to provide an important scientific basis for the realization of the harmless treatment of oil contaminated sludge and the comprehensive utilization of agricultural waste.