石油污染土壤的生物修复过程是一项涉及污染物特性，生物生态结构和环境条件的复杂系统工程，目前对这一过程的机理研究已成为国内外的热点之一。陇东油田分散式分布于农区，其原油的母质类型、本源微生物群落结构、植物具有鲜明区域性，本研究拟以陇东黄土塬为研究靶区，应用地球化学、微生物学、土壤学和植物学等手段，采用本源优势微生物菌群，通过"指纹分析"研究不同菌株对石油各组分的不同作用特点，利用生物标志物的演化参数揭示微生物降解石油的演变规律；将优势菌群投入当地根系发达的植物土壤中，研究不同植物-微生物根系部位在不同程度污染土壤中的微生物群落多样性信息，跟踪降解菌群的定植及发挥作用的过程，寻找植物根际-微生物-土著菌群的相互作用及变化；分析环境中pH、含水量、易溶盐、氮磷等水平对修复作用的影响，从而揭示植物-微生物修复石油污染土壤作用的生物-化学机理。研究结果将为陇东石油开发中的污染治理提供科学依据。

Bioremediation of petroleum contaminated soil is a complex engineering involving pollutants characteristics, biological and ecological structure and environmental conditions, at present, studying mechanism of this process has become one of the hot topics at home and abroad. Oil wells disperse in rural areas in Longdong aera, crude material types, original microbial community structure, plant has distinct regional. The study intends to Longdong Loess Plateau as the research target area, on the basis of Geochemistry, Microbiology, Soil and Botany, the original dominant bacteria has been constructed, the different characteristics of petroleum fractions by different strains' degradation will be exploited through the "fingerprint analysis", the evolution law of microbial degradation to petroleum will be revealed through the evolutional parameters of the biomarker. The dominant bacteria will be added into the local vegetable soil with developed root, information of microbial species diversity of different plant roots will be studied in different degree polluted soils，tracking the colonization and process of bacteria playing a role, interactions and change law among plants' root system ,rhizosphere microbial and indigenous microbe groups will be also researched; effects of environmental factors on the remediation will be analysized such as pH, moisture content,soluble salt, nitrogen and phosphorus' levels.So the work will reveal the mechanism of plant - microbial bioremediation to petroleum contaminated soil. It is expected to provide a scientific basis for the pollution control of oil development in the east area of Gansu.