急性ST段抬高心梗（STEMI）患者行直接冠脉介入治疗后造影剂所致急性肾损伤（CI-AKI）发生率高，且增加近期和远期不良心肾事件。肾小管上皮细胞的自噬在AKI的发生起着重要作用；同时，我们前期研究发现CI-AKI组的自噬相关蛋白beclin-1表达升高，LncRNA XLOC_036125和与之空间位置相邻的Lgmn表达下降，而Lgmn与beclin-1功能相似（GO分析）。然而，尚不明确LncRNA是否通过Lgmn调控自噬而影响CI-AKI发生。本课题组拟在前期研究基础上探讨（1）肾小管上皮细胞中LncRNA对Lgmn调控作用（2）LncRNA靶向Beclin-1对肾小管细胞及模拟STEMI大鼠CI-AKI自噬的调控网络（3）STEMI患者血标本验证XLOC_036125–Lgmn- Beclin-1自噬轴。

Contrast induced acute kidney injury (CI-AKI) is one important complication of ST-segment elevation-myocardial infarction (STEMI) patients after primary percutaneous coronary intervention, and it increased the risk of short- and long-term cardiac and renal adverse events. Autophagy plays an important role in AKI development. Our previous studies demonstrated that the expression of autophagy related protein-Beclin-1 is high in CI-AKI rats; lncRNA XLOC_036125 and Lgmn which be located the adjacent its space position were significant lower in CI-AKI group. Furthermore, the Lgmn gene was similar to beclin-1 in the GO analysis. However, it remains unclear whether LncRNA influences the CI-AKI development through mediating autophagy by Lgmn in renal tubular epithelial cells. Based on our previous results, we aimed to investigate: (1) Verifying the regulating effect of lncRNA XLOC_036125 targeting Lgmn and the effect of lncRNA targeting lgmn on autophagy in the CI-AKI renal tubular cell；(2)Investigating the molecular network mechanism of LncRNA-beclin-1 axis in the close to STEMI animal models and renal tubular cells.(3) verifying LncRNA - Lgmn- beclin-1 autophagy axis in STEMI patients.