目前, 基于生物质谱技术与shotgun策略的糖蛋白质组学技术，蛋白质/肽段的质谱前分离富集已经成为瓶颈问题。亲水色谱法具有操作简单、广谱富集和良好的质谱兼容性等优势，但亲水色谱法面临亲水基质种类少、制备方法复杂等制约，探究制备方法操作简单、性能良好亲水基质是需要解决的关键问题。本课题旨在结合磁性分离与亲水色谱的优点，以亲水性能好、价格便宜、生物兼容、环境友好的糖类化合物为功能单体，以纳米磁球为核心，采用操作简单的蒸馏沉淀聚合与点击化学方法，制备性能优良的糖基亲水性磁性复合物，将其用于糖蛋白组学的分析研究，探究糖基亲水磁性复合物亲水富集分离糖蛋白/糖肽的作用机理，建立基于糖基亲水材料亲水色谱富集分离-LC-MSMS的HepG2细胞糖蛋白组学分析方法，为糖蛋白组学分析方法提供新的思路。

At present, it is the key factor to separate and enrich proteins/peptides prior to mass spectrometry for glycoproteomics analysis based on the strategy of biological mass spectrometry technology. Enrichment by hydrophilic chromatography has the advantages of simple operation, global enrichment and good compatibility with the mass spectrum, but lack of favorable matrix and facilely preparation method have impeded its application. It is vital to develop simple preparation method for fabricating superior hydrophilic matrix. This project plans to prepare glyco-hydrophilic magnetic composites using cheap, environment friendly, well hydrophilic and biocompatible carbonhydrate as the functional monomer and nano magnetic ball as the core by simple distillation precipitation polymerization and click chemistry. Combining the advantages of magnetic separation and hydrophilic chromatography, the hydrophilic magnetic composites would be applied to glycoproteomics, and the mechanism of enrichment by hydrophilic interaction chromatography would be explored. The glycoproteomics analysis method of HepG2 would be developed based on hydrophilic interaction chromatography and LC- MSMS. It would be a new perspective for the research of glycoproteomics.