创伤微环境HGF/c-Met信号促进鹿茸间充质干细胞迁移、增殖和分化的调控机制

创伤对鹿茸的再生与发育均有明显促进作用，且修复再生过程几乎没有炎症反应；创伤微环境间充质干细胞迁移、增殖与分化活动是哺乳动物组织修复、器官再生的研究热点，肝细胞生长因子HGF/c-Met信号通路是已知的创伤微环境唯一的非炎症反应下介导干细胞迁移归巢、增殖与分化的关键信号通路。以创伤再生鹿茸为材料通过实时定量PCR、基因过表达及基因敲除和细胞移植的方法，研究创伤后鹿茸间充质干细胞的微环境变化和非炎症反应及HGF/c-Met信号对鹿茸间充质干细胞系列活动的影响，进一步阐明鹿茸间充质干细胞的特性，从试验上证明创伤后HGF/c-Met信号通路可以介导鹿茸间充质干细胞迁移归巢、增殖与分化，从分子水平揭示创伤微环境对鹿茸组织再生与发育的促进作用机制，完善鹿茸再生理论。

Mechanical injury has a significant effect on the regeneration and development of antler, and almost no inflammation reaction is present in the wound healing process. The migration, proliferation and differentiation of mesenchymal stem cells in wound microenvironment are the focus of wound healing and regeneration studies in mammalian. Hepatocyte growth factor (HGF/c-Met) signaling pathway is the key signaling pathway of stem cell migration, homing, proliferation and differentiation, which is known as the only non-inflammatory response in wound microenvironment.Using the method of real-time quantitative PCR, gene over-expression, gene knockout and cell transplantation,the study of the influence of microenvironment, non-inflammatory reaction and HGF/c-Met signal on antler mesenchymal stem cells after mechanical injury will clarify the characteristics of the antler mesenchymal stem cells. The study would be helpful to prove the wound can mediate the migration and homing, proliferation and differentiation of stem cells in non-inflammatory response through HGF/c-Met signaling pathway, and it would also be useful at molecular level to reveal the promoting effect of trauma on the tissue regeneration and redevelopment of antler, which could make the theory of deer antler regeneration more perfect.