Differential Transcriptome Analysis of Cervus elaphus songaricus and Cervus elaphus yarkandensis Reveals Candidate Genes for Antler Regeneration
Differential Transcriptome Analysis of Cervus elaphus songaricus and Cervus elaphus yarkandensis Reveals Candidate Genes for Antler Regeneration
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马鹿和叶尔羌马鹿的差异转录组分析揭示了鹿角再生的候选基因
DOI：
10.1134/s1022795420030187
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发表时间：
2020-03
影响因子：
0.6
通讯作者：
Ren Z
中科院分区：
文献类型：
--
作者：
Zhao P;Wang S;Ma Z (Ma;Han C;Ren Z
鹿角作为一种中药具有很高的药用价值，具有很高的经济价值。松香鹿茸和yarkandensis Cervus elaphus都是中国yarkandensis的重要品种，但它们的鹿角生产水平不同。因此，从冷冻鹿角组织中提取总RNA并进行RNA测序。RNA-seq数据用多种生物信息学软件处理。采用q-PCR检测差异基因的表达情况。根据鹿角的RNA-seq数据，采用de novo组装方法获得385,712个unique基因，并在Nr、Swissprot、GO和KEGG等数据库中进行了注释。结合DGE技术，检测宋菖蒲和雅kandensis的转录组差异（>；±2倍，p值<；0.05）。共鉴定出上调基因20个，下调基因27个，其中7个基因（CYTB petB、COX7B、EPHX2、KCNQ2、CCLX、RP-S11e和RPS11）被定位到14条信号通路。yarkandus elaphus yarkandensis具有较高的代谢水平、较快的细胞增殖速度和较强的免疫力，从而提高了鹿茸产量。本研究通过对鹿角转录组的比较研究，发现了一些重要的差异表达基因和信号通路，这可能解释两种鹿角再生的差异，并为进一步了解鹿角产生的分子机制提供有益的信息。
Antler is of high medicinal value as a traditional Chinese medicine and it has a high economic value. Both Cervus elaphus songaricus and Cervus elaphus yarkandensis are important species of Cervus elaphus yarkandensis in China, but they are different in the level of deer antlers production. So total RNA was extracted from frozen antler tissue and performed RNA-seq. Data from RNA-seq was disposed with multiple bioinformatics software. The expression of differential genes were evaluated by q-PCR. Based on the RNA-seq date of antlers, we obtained 385,712 unigenes by employing de novo assembly and then they were annotated in some databases, such as Nr, Swissprot, GO and KEGG. Combined with DGE technology, transcriptome differences between Cervus elaphus songaricus and Cervus elaphus yarkandensis were detected (>±2 Fold, P-value <0.05). There were altogether 20 up-regulated and 27 down-regulated genes identified, among which 7 (CYTB petB, COX7B, EPHX2, KCNQ2, CCLX, RP-S11e and RPS11) were mapped to 14 signaling pathways. The Cervus elaphus yarkandensis had higher metabolic levels, faster cell proliferation, and stronger immunity, which improved the yield of antler production. This comparative study of the antlers transcriptome between Cervus elaphus songaricus and Cervus elaphus yarkandensis identified some important differentially expressed genes and signaling pathways, which may explain the differences of two species antler regeneration and providebeneficial information for further understanding of the molecular mechanism of Cervus elaphus antlers production.
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影响因子：
5.8
作者：
Tong, Mark Y.;Cassa, Christopher A.;Kohane, Isaac S.
通讯作者：
Kohane, Isaac S.
影响因子：
46.9
作者：
Trapnell C;Williams BA;Pertea G;Mortazavi A;Kwan G;van Baren MJ;Salzberg SL;Wold BJ;Pachter L
通讯作者：
Pachter L
影响因子：
4.4
作者：
Guo Q;Ma X;Wei S;Qiu D;Wilson IW;Wu P;Tang Q;Liu L;Dong S;Zu W
通讯作者：
Zu W
影响因子：
2.6
作者：
J. Mikler;C. Theoret;Jerry C High
通讯作者：
J. Mikler;C. Theoret;Jerry C High
DOI：
10.1007/s004290100204
发表时间：
2001-11-01
期刊：
ANATOMY AND EMBRYOLOGY
影响因子：
--
作者：
Li, CY;Suttie, JM
通讯作者：
Suttie, JM