PM2.5 诱发的氢氧自由基（oOH）与过渡金属和醌类相关。为了理解实际PM2.5诱导生成的oOH，本课题将分别采用模型化合物和实际PM2.5来模拟在人工肺流体中产生的oOH。模型化合物选择Fe,Cu和醌类(1,2-萘醌, 1,4-苯醌, 9,10-菲醌），按照不同的组合方式，量化过渡金属与醌类联合诱导生成oOH的协同作用。实际PM2.5选择过渡金属和醌类有明显差别的四类主要贡献源（汽油车尾气、柴油车尾气、燃煤电厂和生物质燃烧），模拟在人工肺流体中诱导产生的oOH，对PM2.5中过渡金属和醌类进行成分分析，并与实验室模拟结果进行比对，探讨PM2.5诱导产生oOH的影响因素；该课题可以了解PM2.5的毒性成分和特征，有助于理解PM2.5致机体损伤的的毒理作用,更好地为预防疾病发生、保护人类健康提供科学依据，并且对环境保护部制订控制PM2.5主要污染源的方案提供技术支撑。

As the most reactive and toxic reactive oxygen species (ROS),oOH was related to many pathonogenetic and toxicological pathways. Although there is increasing recognition that transition metals and quinones can play important role in oOH generation for several years, unfortunately, the mechanism of how PM2.5 induced oOH generation remains unclear. In this project, Fe,Cu and quinones (1,2-naphthaquinone,1,4- benzoquinone, 9,10-Phenanthrenequinone) will be chosen as modulate to simulate the mechanism of oOH generation, PM2.5 will be collected from four main contributed sources (gasoline exhaust, diesel exhaust, coal-fired power plant and biomass burning), which has different value of transition metals and quinones. We will simulate the oOH generation in cell-free surrogate lung fluid, analyze the concentration of transition metals and quinones in PM2.5 and compare the actual PM2.5-induced oOH with the lab simulation results, so as to quantify oOH generation which is synergistically induced by combined transition metals and quinones,and further discuss the influencing factor of PM2.5-induced oOH generation. This project will provide the pollution characteristics and toxicity of fine particles,scientificly support the disease prevention and human health protection and technically support PM2.5 control plan for Ministry of Environmental Protection.