Miniature Inverted-repeat Transposable Elements 是一类重要的转座子，已有的研究普遍推测MITEs扩增和活动在基因组进化中起到重要的作用，并影响附近基因的表达。MITEs在芸薹属各物种基因组中分布广泛，但鲜见其在芸薹属中活动的研究，MITEs对基因表达的影响也多是推测，缺乏充分的实验支持。本研究，利用MITEs拷贝数多寡不同的人工合成甘蓝型油菜及其亲本，综合利用高通量测序及分析、基因表达、表观遗传修饰和遗传转化等技术，研究白菜和甘蓝相互杂交形成甘蓝型油菜的过程中，MITE因子Monkey King的活动规律，解析Monkey King在介导邻近基因表达调控中所起的作用及分子机理。这一研究不仅有助于揭示芸薹属基因组多倍化过程中转座子对基因组结构和基因功能的影响，还有助于利用MITEs活动造成的物种内和物种间的插入/缺失多态性进行芸薹属作物遗传改良

Miniature Inverted-repeat Transposable Elements are important transposable elements, and predicted to play important roles on genomic evolutionary and gene expression. Though MITEs are widespread in Brasscia genome, studies about activities of MITEs in Brassica genomes are rare and theories about MITEs regulating gene expression are lack of solid experimental supports. In our project, two synthesized B. napus lines with different MITEs density in their genomes and their diploid parents (B. rapa and B. olerecea) are used as materials. We aim to elucidate activities of transposable element, Monkey King, and it induced neighboring gene regulation in Brassica species through high-throughput sequencing and bioinformatic analysis, transcriptome sequencing, epigenetic analysis and transformation technologies. This research not only contribute to demonstrate the impact of transposable elements on genomic structure and gene functional divergence during polyploidization, also conduce to genetic improvement through utilizing MITEs induced polymorphism in Brassica species.