肥胖已成为严重威胁人类健康的十大疾病之一，如何有效减少和控制肥胖已成为当前面临的重要科学研究课题。从中药等天然药物中探寻作用于外周抑制脂肪酶吸收和降低氧化应激水平这两个靶点的减肥降脂药物，具有西药无法比拟的安全性和有效性。因此，亟需建立一种与生物活性直接相关的筛选和评价方法，从中药中快速分离、定位和鉴定减肥降脂活性成分。鉴于此，本课题创新性将薄层生物自显影技术与原位电离质谱联用，充分将薄层生物自显影从复杂体系中直观分离、锁定和评价活性成分的特点与原位电离质谱直接解吸活性斑点结构的特点有机结合，通过建立抑制脂肪酶和清除羟自由基活性的新型薄层色谱生物自显影法，以具有显著减肥降脂作用的中药枸骨叶和荷叶等为研究对象，建立基于薄层生物自显影-原位电离质谱联用方法。该方法的建立及应用将为中药减肥降脂活性成分的快速筛选和鉴定，以及建立与药效相关品质评价体系提供新的思路和新的方法。

According to the World Health Organization, obesity is becoming one of the top ten threats to human health. China had surpassed US as the largest population of obese since 2009. Therefore, anti-obese or regulating body weight is a hot topic area of scientific research. It was highly valuable to discover and develop the medications that affect on peripheral inhibition of lipase absorption and HO radicals scavenging from traditional Chinese Medicines (TCMs) and natural sources. Due to the complexity of TCM constituents, it is necessary to establish a new bio-analytical method that is directly associated with pancreatic lipase inhibition and HO radical scavenging. The thin-layer chromatography (TLC)-bioautography method is an internationally recognized approach that combines the TLC separation and in situ determination of bioactivity. In this proposal, a totally new TLC-bioautography-ambient mass spectrometry method is to be developed for simultaneous separation, screening, identification of the potential pancreatic lipase inhibitors and HO radical scavengers from Ilex cornuta and Nelumbo nueifera that were previously confirmed with the activity of regulating body weight. The successful development of proposed the TLC-bioautography-ambient mass spectrometry method in this project would provide a new thought and method for fast separation, screening, identification, and quality evaluation of TCMs, which is directly associated with their bioactivities.