碳源缺乏是目前困扰城市污水处理脱氮除磷的核心问题之一。外碳源丰富-贫乏的污水运行模式可诱导微生物产生体内储能聚合物积累/分解的代谢响应。申请人拟将这一代谢行为与污水中氮的去除偶联，使其中的部分有机物通过细胞体内聚合物过量累积以去除，累积的聚合物进一步作为后续反硝化电子供体，从而实现细胞聚合物驱动的反硝化生物脱氮。该过程有望解决生物脱氮碳源缺乏的问题，还可同步降低去除有机物所需能耗，因而极具应用潜力。课题拟对这一过程开展基础科学研究，融合水处理工程学、微生物学及生物化学等多学科的理论与分析方法，探寻细胞聚合物形成模式及形成种类与碳源类型的关系，研究该过程微生物代谢特征及响应、细胞聚合物形成/分解与氮元素去除的代谢偶联机制，获得细胞聚合物驱动反硝化的优化控制条件，并建立定量表达的脱氮预测模型。通过揭示微生物内聚物驱动反硝化的作用机制，丰富现有的生物脱氮理论，并为进一步工程应用提供科学依据。

Organic carbon source deficiency is still one of the core problems for nutrient removal in an activated sludge system. When microorganisms in this system cultivated through alternative famine/feast period, a metabolic response of microorganisms can be induced to accumulate / decompose cell energy storage polymers with the existence of external carbon source. Based upon this fact, the applicant intends to couple the removal process of nitrogen with the removal of organic pollutants, during which some parts of organic carbon can be converted and accumulated in the form of cell polymers, subsequently, those cell polymers were used as electron donor for denitrification process, thus realize a denitrification process driven by cell polymers. ..The process could be expected to solve the problem of the lack of carbon source for biological denitrification, and meanwhile could reduce the energy consumption as some of organic matters has been converted, which was of great application potential...To start some basic research concerning the mentioned above, an integration technology of multi-disciplinary theory and analysis methods for wastewater treatment engineering, microbiology and biochemistry might be employed, and our main objectivies are to explore the formation and types of cell polymer related to different organic carbon source, and to investigate the process characteristics and response of microbial metabolism, as well as metabolic coupling mechanism of cell polymer formation / decomposition and nitrogen removal...The conditions for cell polymer-driven denitrification will be also optimized, and a model for the process constructed. ..With the research efforts on those, a further understanding could be realized, which would make contributions for and enrich the existing theory of biological nitrogen removal, and provide fundmental knowledge for potential engineering applications.