家蚕与野外昆虫间微孢子虫交叉传染性及其传染机制

家蚕微粒子病是养蚕业中最为严重的一种疫病，该病病原来源复杂，很多研究认为野外昆虫微孢子虫对家蚕的交叉传染是目前蚕种生产中微粒子病发生率上升的重要原因，但一直缺乏确切证据证实两者的相关性。本项目以感染家蚕的异型微孢子虫和野外昆虫微孢子虫为研究对象，采取病原分类分析不同微孢子虫近缘关系和生产环境分布调查相结合分别探讨广西蚕区异型微孢子虫的来源和野外昆虫微孢子虫的传染问题，并在不同环境下调查家蚕与野外昆虫间微孢子虫的交叉传染性。通过上述研究，查找在广西蚕种生产中存在流行趋势的异型微孢子虫，为蚕种微粒子病检疫合理判断提供依据；查找在广西蚕区与家蚕存在交叉传染微孢子虫的主要昆虫，为蚕业生产采取合理措施防控家蚕微粒子病提供依据；探讨家蚕与野外昆虫间微孢子虫交叉传染机制，为建立阻断野外昆虫微孢子虫对家蚕交叉传染有效途径提供科学依据和理论参考，也为生物界防控微孢子虫病发生提供借鉴。

Silkworm pebrine was the most serious epidemic disease for sericulture, the pathogen source was complex. Many studies suggested that the increased incidence of silkworm pebrine in silkworm egg production was because of microsporidia cross-infection between wild insects and silkworm, but the definite evidence was lack for that correlation. This project take heterotypic microsporidia and insect microsporidia as research objects, then research the origin of heterotypic microsporidia and the infection of insect microsporidia by the ways of relation- ships analysis with pathogenic classification and investigation on the pathogen distribution, and research for the microsporidia cross-infection between silkworm and wild insects under different environment. Therefore, the project search the epidemic species of heterotypic microsporidia in silkworm egg production, that will provide the basis for reasonable judgment of silkworm egg quarantine; and search the main species of insect microsporidia which has cross-infection to silkworm in Guangxi sericulture area, that will provide the basis for make reasonable measures to control silkworm pebrine. The project research on the mechanism of cross-infection between silkworm and wild insects, which will provide a scientific basis and theoretical reference to establishment of an effective way to control the microsporidia cross-infection between wild insects and silkworm, and provide reference to control microsporidia disease in the biological world.