除草剂苄嘧磺隆的大量使用，污染了农田土壤，对后茬敏感作物易造成药害。苄嘧磺隆残留在土壤中的降解与微生物密切相关，了解微生物在这一过程中的作用对于污染农田土壤的修复具有重要意义。本项目以申请者分离的苄嘧磺隆降解菌株Hansschlegelia zhihuaiae S113为材料，研究其在玉米根际土壤中降解苄嘧磺隆残留的机制。拟解析接种降解菌株对玉米根际土壤苄嘧磺隆残留降解的微生态效应；参与降解苄嘧磺隆残留的土著微生物种类；降解菌株在玉米根际的定殖、存活与生物膜的形成；降解菌株对玉米根系分泌物和苄嘧磺隆残留的趋化响应及差异；趋化基因cheA在降解菌株生物膜形成和苄嘧磺隆残留降解过程中的作用等内容。研究结果可进一步阐明玉米根际土壤苄嘧磺隆残留的微生物降解机制，为除草剂苄嘧磺隆残留污染农田土壤的修复提供更多的理论依据。

Bensulfuron-methy has been widely used in China. The residues of this herbicide have contaminated agricultural soil, and have exhibited phytotoxicity on rotation sensitive crops. The degradation process of herbicides residues in the agricultural soil is closely related to the functional microorganisms. Understanding the role of bacteria in the degradation process is of a great significance for the bioremediation of herbicides contaminated soil. The degrading strain Hansschlegelia zhihuaiae S113, which was isolated by our lab, was used to study the degradation mechanism of bensulfuron-methy residues in the rhizosphere of maize. In this study, we will investigate the effects of microbe ecology，and identify the species involved in the bensulfuron-methy degradation in the rhizosphere of maize. After inoculation of strain S113, we will observe the colonization of strain S113 and the formation of biofilms in the rhizosphere of maize, and elucidate the strain’s different responses to the maize root exudates and bensulfuron-methy residues. Further, we will identify the role of cheA in the chemotaxis, the formation of biofilm and the degradation of bensulfuron-methy residues. This research will reveal the mechanism of degradation of herbicides residues in the rhizosphere of maize, and provide theoretical instructions for bioremediation of herbicides contaminated agricultural soil.