玻璃化一直被认为是生物材料长期低温保存效果最好的方式，但它目前仅成功应用于小型生物系统，而对于较大型玻璃态组织或器官（特征尺寸>1mm），则由于传统复温方式的局限性，使得其至今没有得到成功应用。本项目提出利用中低频交变磁场（频率<1MHz）诱导均匀分布在玻璃态生物材料中的磁纳米粒子进行加热，有望实现快速而均匀的加热目的，从而消除较大体积玻璃态生物材料复温时伴随的反玻璃化损伤和热应力损伤，使得生物材料在复温过程中保持细胞和宏观结构的完整性。项目拟探索三个方面的基础性关键问题：（1）磁纳米粒子在低温保护剂和生物材料体系中的加载方法和分布均匀性；（2）这三者复合体系在冻融过程中的物理化学特性；（3）较大体积玻璃态生物材料磁热复温效果的定量评估方法。该项目的实施有利于寻求较大型玻璃态组织器官的理想复温控制方法，对捐献组织器官的长期贮存和临床应用具有重要意义。

So far, vitrification is still thought as the best form for long-term storage of cryopreserved biomaterials. Unfortunately, practical application of vitrification has been limited to smaller systems and due to some limitations of conventional heating methods those preclude its use in larger bulk organs and tissues (the characteristic size is more than 1mm). To circumvent this problem, we propose here to use low-medium frequency (less than 1MHz) alternating magnetic field (AMF) to excite magnetic nanoparticles (mNPs) uniformly distributed within vitrified biomaterials. This approach can be expected to obtain faster and more uniform heating rates, which has the potential to dramatically eliminate devitrification and thermal stress associated cracking to improve viability and structural integrity upon heating. In current proposal, three critical issues such as the distribution characteristics of mNPs loaded into both cryoprotective agents (CPAs) and biomaterials, the physicochemical properties of the mixed systems of mNPs+CPAs+biomaterials upon freezing and heating as well, and the quantitative assessment methods for mNPs heating by AMF with experiments and theoretical models, will be explored to optimize the performing system(mNP, CPA and applied field parameters) for successfully fast heating of larger bulk vitrified biomaterials. Importantly, the research proposed here will be very helpful for long-term storage of donated orgains and clinical application as well.