静电纺胶原基纳米纤维仿生制备组织工程支架是生物材料领域的研究热点和难点。相关研究仍存在胶原结构稳定性与静电可纺性的矛盾问题，且未明确纺丝液中胶原聚集行为与电纺纤维性质的关系。本项目基于疏水缔合作用耐温、耐剪切及其利于胶原电纺的优势，提出“先改性，再纺丝”创新思路，旨在通过疏水改性同步增强胶原结构稳定性与静电可纺性，实现对电纺胶原纤维性质的调控。拟制备新型脂肪酸-硫代NHS酯对鱼胶原进行疏水改性，采用热动力学、二维相关分析、流变等技术阐明改性鱼胶原稳定性与静电可纺性的同步增强机制；揭示电纺液中改性鱼胶原聚集行为与电纺纤维性质的内在关联；掌握脂肪酸-硫代NHS酯疏水改性对电纺鱼胶原纳米纤维构效关系的调控机理。本项目对减轻电纺溶剂、温度和剪切力对胶原的协同变性作用及发展胶原电纺用水性溶剂体系，都有重要理论意义和应用价值，也为促进鱼胶原的改性与应用以及推动鱼下脚料资源高值转化利用进程提供理论依据。

The electrospinning of collagen-based nanofibers to prepare scaffolds for tissue engineering is a hot and difficult issue of research in the field of biomaterials. There remains a problem of contradiction concerning the structural stability of collagen and its electrospinnability. Additionally, the correlation between the aggregation behavior of collagen in the electrospinning solution and the structure-property of electrospun nanofibers has not been clarified. Based on the fact that hydrophobically associating interactions are resistant to temperature and shear force and are beneficial to the electrospinning of collagen, this project proposes a novel idea about "Modification first and then electrospinning" and aims to synchronously strengthen the structural stability of collagen and its electrospinnability, achieving the regulation of the structure-property of electrospun collagen fibers. The project is to prepare novel fatty acidic N-Hydroxysulfosuccinimide esters to hydrophobically modify fish collagen and the mechanism of the synchronous enhancement of the structural stability and the electrospinnability of the modified collagen will be disclosed using several techniques including thermal kinetics, two-dimensional correlation analysis, rheology and so on. Moreover, the internal relations between the aggregation behavior of the hydrophobically modified fish collagen in the electrospinning solution and the structure-property of electrospun nanofibers will be clarified. Finally, the mechanism of the regulation of the structure-property of electrospun collagen nanofibers through the hydrophobic modification of fish collagen by fatty acidic N-Hydroxysulfosuccinimide esters will be mastered. The project has important theoretical significances and application values for reducing the cooperative denaturation effects on collagen of electrospinning solvents, temperature and shear force, and for developing water-based solvent for collagen electrospinning. And the project will provide theoretical basis for the promotion of the modification and application of fish collagen, as well as the high-value utilization of fish offal resources.