血管通透性增高是严重创伤/休克后的重要病理生理改变，但机制远未阐明。基于天然免疫系统早期激活是导致后期复杂临床表现重要原因的最新认识，根据前期研究发现创伤/休克后肺IL-33-ILC2信号轴活化，启动早期2型免疫反应，以及文献报道2型细胞因子IL-5可调节血管通透性，推测天然免疫系统IL-33-ILC2信号轴活化可能促进创伤/休克后的血管通透性增高。本研究拟采用C57BL/6小鼠、以及骨髓重建获得的ILC2 KO小鼠，复制严重创伤/休克模型，采用流式细胞、免疫荧光、离体细胞和整体动物水平的血管通透性检测等技术，观察：1）IL-33-ILC2信号轴在严重创伤/休克后肺血管通透性增高中的作用；2）IL-33-ILC2轴启动早期2型免疫反应后，是否通过促进VEGF和MMP-9表达，降低VE-Cad，开放内皮细胞间途径，导致血管通透性增高。以期从新的角度阐明严重创伤/休克血管通透性增高的启动机制。

Vascular hyperpermeability is an important pathological change after severe trauma/shock, while its mechanism is still unclear. Based on the the latest opinion that the early activated innate immune system could lead to the later complicated clinical trajectories; and our preliminary study which showed the interleukin-33 (IL-33)- type II innate lymphoid cells (ILC2) signal axis of the innate immune system was activated and triggered the early type 2 immune response after severe trauma/shock; as well as the previous researches which reported the regulatory effect of type 2 cytokine IL-5 on vascular permeability, so we presumed that the IL-33-ILC2 signal axis of the innate immune system could be activated and contribute to the vascular hyperpermeability after severe trauma/shock. To verify the hypothesis, the C57BL/6 mice and ILC2 KO mice set up by bone marrow reconstituting were used to make the severe trauma/shock model, and the flow cytometry, immunofluorescence, vascular permeability detection technique in vivo and in vitro were adopted to observe: 1) Role of IL-33-ILC2 signal axis in the vascular hyperpermeability in lung after severe trauma/shock; 2) If IL-33-ILC2 signal axis activation and the early type 2 immune response lead to the vascular hyperpermeability after severe trauma/shock by promoting the expression of vascular endothelial growth factor (VEGF) and activation of matrix metalloproteinase-9 (MMP-9), decreasing the expression of vascular- endothelial cadherin (VE-Cad), and opening the paracellular pathway. With this research, we hope to illuminate the startup mechanism of vascular hyperpermeability after severe trauma/shock with a new view.