红麻雄性不育系的发现推动红麻产业的发展，但对不育原因的分子研究进展缓慢。我们培育了红麻雄性不育系，前期细胞学形态观察研究表明雄性不育花粉外壁发育异常。以红麻LC0301A和LC0301B为对象，高通量转录组测序技术极大提高了筛选和鉴定花粉壁发育相关的差异表达基因的效率。通过创建相关基因的超表达和 RNA 沉默表达载体，获得性状稳定的转基因植株。利用亚细胞定位、Southern和Northern技术对花粉壁相关基因时空表达模式进行探究;从而分析其在花粉发育进程中的细胞外壁的变化情况，了解红麻雄性不育的败育进程;明确控制花粉壁发育基因对红麻花粉壁的调控作用，为红麻花粉壁的发育提供新的理论依据。探讨控制花粉壁发育基因是否是决定红麻雄性不育的关键因子；寻找通过基因工程技术培育红麻雄性不育系的新途径。

Discovery of Kenaf male sterility promotes the development of Kenaf Industry, but systematic molecular research of infertility is slow. We have bred kenaf male sterility. It showed that male sterile pollen extine became abnormal in early cytological observation. With LC0301A and LC0301B , high-throughput transcriptome sequencing technology greatly improves the efficiency of gene expression and development of screening and identification of pollen wall related differences . Through over expression and RNA silencing techniques, we construct vectors to create related genes and obtain transgenic plants with stable traits. The characteristics of phenotype and mechanisms of physiology and biochemistry will be studied in the control and transgenic plants with the subcellular localization, Southern and Northern technology. Changes in pollen wall development process is to elucidate the abortion process of male sterility of kenaf. Regulation of the explicit control of pollen wall growth gene provides a new theoretical basis for the development of the exnine. To investigate whether the control of pollen wall development gene is the key factor to affect the male sterility of Kenaf by new ways. It will provide with genetic engineering techniques for the breeding of Kenaf male sterility.