研究表明，miR-26a表达水平与精子活力相关。前期工作发现，miR-26a可直接靶控PDHX表达影响精子活力，与精子代谢相关。PDHX编码丙酮酸脱氢酶复合体PDHc中的E3BP亚基，在催化丙酮酸脱羧成为乙酰辅酶A过程中扮演关键角色，连接糖酵解和后续柠檬酸循环和氧化磷酸化途径。miR-26a/PDHX途径影响精子代谢和活力及其机制的研究未见报道。本研究在前期工作基础上进一步检测miR-26a/PDHX在附睾不同部位和不同保存条件精子中的表达、miR-26a/PDHX甲基化模式对猪精子代谢和活力的影响，阐明猪精子常温保存过程中的代谢途径。研究的结果将为进一步揭示猪精子代谢模式和途径、改善和优化猪精液稀释液从而提高猪受胎率和窝产仔数等具有重要的理论和实践参考价值。

It was reported that miR-26a is associated with sperm motility. Our previous works have found that miR-26a can directly bind to 3'-UTR of PDHX, and affect PDHX expression and sperm motility, which is speculated to be involved in sperm metabolism. PDHX, encoded E3BP subunit of pyruvate dehydrogenase complex (PDHc), plays a key role in catalyzing pyruvate into acetyl-CoA, and links glycolysis and subsequent citric acid cycle and oxidative phosphorylation pathway. The effects and mechanism of miR-26a/PDHX pathway on sperm metabolism and motility remain unknown. Thus, based on our previous works, we will further determine the expression level of miR-26a/PDHX in sperm with different location of the epididymis and under different liquid storage conditions. Then, the methylation patterns of miR-26a/PDHX and interaction with boar sperm metabolism and sperm viability will be further explored to reveal the metabolic pathway of porcine sperm. The results will further benefit to clarify the pig sperm metabolism pathway, and provides important theoretical and practical value for improvement and optimization of pig semen liquid storage or freezing extender to improve the farrowing rate and litter size after artificial insemination..