Comparative Analysis of piRNA Profiles Helps to Elucidate Cryoinjury Between Giant Panda and Boar Sperm During Cryopreservation.
Comparative Analysis of piRNA Profiles Helps to Elucidate Cryoinjury Between Giant Panda and Boar Sperm During Cryopreservation.
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piRNA谱的比较分析有助于阐明大熊猫和公猪精子在冷冻保存过程中的冷冻损伤
DOI：
10.3389/fvets.2021.635013
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发表时间：
2021
影响因子：
3.2
通讯作者：
Zeng C
中科院分区：
文献类型：
--
作者：
Wang Y;Zhou Y;Ali MA;Zhang J;Wang W;Huang Y;Luo B;Zhang H;Qin Z;Zhang Y;Zhang M;Zhou G;Zeng C
冷冻可引起精子冷冻损伤，包括生理和功能的变化。然而，精子冷冻损伤和冷冻耐受性的分子机制仍不清楚。精子的耐冷冻性或冷冻耐受性因物种而异，野猪精子更容易受到冷应激的影响。与公猪精子相反，大熊猫精子似乎具有很强的冷冻耐受性，能够在反复冻融循环中存活。本研究对大熊猫和野猪新鲜精子和冻融精子中具有不同冷冻耐受能力的PIWI相互作用RNA（piRNA）进行了差异表达分析。结果显示，在大熊猫和野猪精子中分别鉴定出1，160个（22个下调和1，138个上调）和384个（110个上调和274个下调）DE piRNA。基因本体（GO）富集分析表明，DE piRNA的靶DE信使RNA（mRNA）主要富集在大熊猫和野猪精子的生物调节、细胞和代谢过程中。京都基因与基因组百科全书（KEGG）分析表明，DE piRNA的靶基因DE mRNA在大熊猫中仅分布于DNA复制和环磷酸腺苷（cAMP）信号通路，而在猪精子中则被认为是嗅觉信号通路的一部分。综上所述，我们推测大熊猫和公猪精子中cAMP信号通路的皮尔纳谱和DE piRNA的差异可能是导致大熊猫和公猪精子抗冻能力不同的原因，这有助于阐明精子冷冻损伤和抗冻性背后的分子机制。
Cryopreservation induces sperm cryoinjuries, including physiological and functional changes. However, the molecular mechanisms of sperm cryoinjury and cryoresistance are still unknown. Cryoresistance or the freeze tolerance of sperm varies across species, and boar sperm is more susceptible to cold stress. Contrary to boar sperm, giant panda sperm appears to be strongly freeze-tolerant and is capable of surviving repeated cycles of freeze-thawing. In this study, differentially expressed (DE) PIWI-interacting RNAs (piRNAs) of fresh and frozen-thawed sperm with different freeze tolerance capacity from giant panda and boar were evaluated. The results showed that 1,160 (22 downregulated and 1,138 upregulated) and 384 (110 upregulated and 274 downregulated) DE piRNAs were identified in giant panda and boar sperm, respectively. Gene ontology (GO) enrichment analysis revealed that the target DE messenger RNAs (mRNAs) of DE piRNAs were mainly enriched in biological regulation, cellular, and metabolic processes in giant panda and boar sperm. Moreover, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that the target DE mRNAs of DE piRNAs were only distributed in DNA replication and the cyclic adenosine monophosphate (cAMP) signaling pathway in giant panda, but the cAMP, cyclic guanosine monophosphate (cGMP), and mitogen-activated protein kinase (MAPK) signaling pathways in boar sperm were considered as part of the olfactory transduction pathway. In conclusion, we speculated that the difference in the piRNA profiles and the DE piRNAs involved in the cAMP signaling pathway in boar and giant panda may have contributed to the different freeze tolerance capacities between giant panda and boar sperm, which helps to elucidate the molecular mechanism behind sperm cryoinjury and cryoresistance.
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影响因子：
3
作者：
Benavides, Fernando;Sutovsky, Peter;Echevarria, Luisa
通讯作者：
Echevarria, Luisa
影响因子：
3.7
作者：
Fahlgren N;Howell MD;Kasschau KD;Chapman EJ;Sullivan CM;Cumbie JS;Givan SA;Law TF;Grant SR;Dangl JL;Carrington JC
通讯作者：
Carrington JC
影响因子：
3.8
作者：
Chen, Xiaoli;Zhu, Huabin;Wang, Dong
通讯作者：
Wang, Dong
精浆 piRNA 作为男性不育分子生物标志物的系统表征
影响因子：
4.6
作者：
Hong Y;Wang C;Fu Z;Liang H;Zhang S;Lu M;Sun W;Ye C;Zhang CY;Zen K;Shi L;Zhang C;Chen X
通讯作者：
Chen X
影响因子：
4.4
作者：
Capra E;Turri F;Lazzari B;Cremonesi P;Gliozzi TM;Fojadelli I;Stella A;Pizzi F
通讯作者：
Pizzi F