sul基因在猪源大肠杆菌和人源嗜麦芽寡养单胞菌之间转移频率研究

嗜麦芽寡养单胞菌是免疫力低下患者呼吸道、泌尿道、伤口等部位感染的常见病原，严重者出现菌血症及心内膜炎，也是医院感染的常见病原；其对多种抗菌药物存在耐药，磺胺甲噁唑／甲氧苄啶是目前治疗嗜麦芽寡养单胞菌感染的首选药物，但该细菌对磺胺药耐药日趋严重，使其临床感染难以控制。目前国内猪源细菌对磺胺药高水平耐药现象十分普遍，且耐药谱型与人源细菌十分相似。sul基因介导细菌对磺胺类的高度耐药，本研究以猪源大肠杆菌、人源嗜麦芽寡养单胞菌以及细菌sul基因为研究对象，探讨sul基因是否可以从猪源大肠杆菌转移至人源嗜麦芽寡养单胞菌，并推测其转移的频率，为客观评价动物生产中抗菌药使用导致的细菌耐药是否对人健康构成危害及其危害风险提供基础理论支持。

Stenotrophomonas maltophilia was common pathogeny of respiratory tract, urinary tract, and wound infection, and severe cases appeared bacteremia and endocarditis. It was also common cause of nosocomial infection, and it was resistence to antibiotics . Sulfamethoxazole/ trimethoprim is the first choice drug for treanting the infection caused by Stenotrophomonas maltophili. But the infection in the clinical was more and more difficult to be controled when the resistance of Stenotrophomonas maltophili to sulfonamides has become increasingly serious. Now the high resistence levels of the porcine bacteria to sulfonamides was universal phenomenon in china and the antibiotic-resistant profiles of both sources strains were very similar. The sul genes mediated bacteria the height level resistence to sulfonamides. With the swine E.coli, anthropogenic Stenotrophomonas and sul genes as the research tools, this study intended to discusse whether the sul gene was transfered from swine E. coli to anthropogenic Stenotrophomonas maltophilia and how much the transfer frequency of sul gene it were. The research result might be helpful to assess the harmful risk of drug resistance of animal bacterial to human.