腺毛通过合成、释放大量特异性的次生代谢产物，为植物防御昆虫取食及病原微生物侵染提供化学武器，被称为植物“化学工厂”。然而，以激光显微切割（LMD）结合超高压液质联用（UPLC/MS/MS）的分析方法进行腺毛特异性次生代谢产物及其防御功能的研究却很少。迄今为止，国内外对秦巴山特征植物黄鼠狼花腺毛中次生代谢产物及防御功能的研究尚未见报道。前期研究发现该植物腺毛及叶提取物对植食性昆虫棉铃虫均具有显著的拒食活性，同时对3种植物病原菌均显示不同程度的抑制活性。本项目拟采取LMD-UPLC/MS/MS的分析方法对黄鼠狼花腺毛中的次生代谢产物进行研究，并对这些成分进行分离纯化与结构鉴定，借助活性测试及HPLC定量分析，揭示其防御功能，丰富人们对腺毛化学防御的认识。另外，对其叶中次生代谢产物进行系统研究，结合活性测试，期望发现具有应用前景的天然活性物质，为该植物资源的开发利用提供科学依据和参考价值。

Glandular trichomes(GTs) produce and release a wide variety of specific secondary metabolites which are considered as major chemical weapon against herbivore attack, and thus have been regarded as phytochemical factories. However, study on GTs- specific secondary metabolites and their defense through laser microdissection (LMD) coupled with ultra performance liquid chromatography-tandem mass spectrometer (UPLC/MS/MS) have been rarely reported, and the knowledge of the GTs-specific secondary metabolites of Salvia tricuspis Franch, characteristics in Qin Bashan, together with their defense still remained uninvestigated. Our previous study discovered that the extraction of both GTs and leaves of S. tricuspis exhibit significant antifeedant activity against generalist plant-feeding insect Helicoverpa armigera, meanwhile, they also show potent antifungal activity against three phytopathogenic fungi. Therefore, with LMD-UPLC/MS/MS, we investigate the GTs-secondary metabolites of S. tricuspis and elucidate their structure via spectroscopic methods to reveal GTs-based plant defense and develop understanding of GTs-chemical defense in view of the results of activity tests and HPLC quantitative analysis. Moreover, through investigation on the leaves of S. tricuspis has been expected to explore some prospective biological chemicals. Finally, our study would provide theoretic and scientific bases for the utilization of this plant.