休眠的彻底解除、充足的营养供应是牡丹反季节生产中开花质量的保障。然而，内休眠伴随着胼胝质在筛孔和胞间连丝的沉积，从而堵塞了物质运输的通道，限制了营养的供应。低温和赤霉素（GA）可诱导β-l,3-葡聚糖酶的合成，降解胼胝质从而打通物质运输通道。课题组前期通过转录组测序结合表达模式分析克隆了受GA和低温诱导的β-l,3-葡聚糖酶基因（PsBGs），本项目拟通过功能分析、原位杂交和蛋白定位分析等鉴定参与运输通道重新打通的PsBGs基因；测定牡丹花芽休眠解除中胼胝质分布、β-l,3-葡聚糖酶活力和运输通路状态变化，分析其与相关PsBGs基因表达的相关性；进一步，通过缺失分析鉴定PsBGs启动子中的GA响应元件，并筛选、鉴定相关转录因子，解析GA诱导PsBGs基因表达的调控机制。研究结果对深入理解休眠解除中物质运输的调控，解析休眠解除机理具有重要价值，并为牡丹反季节催花生产技术创新提供理论依据。

To break dormancy is the keystone to the anti-season production of tree peony, and together with sufficient nutrient supplement is the guarantee to flowering quality. However, callose deposits both at plasmodesmata (PD) and sieve pore when plants go into dormancy. As a result, all symplasmic pathways are shut down, which will finally reduce flowering quality if they can’t be thoroughly reopened when dormancy release. It was proved that chilling and gibberellins could induce the synthesis of β-l,3-glucanases to degrade callose and recover the symplasmic paths. Previously, we had screened several β-l,3-glucanases genes (PsBGs) induced by chilling and GA, combining transcriptome and expression analysis. In this study, PsBGs genes involved in recruiting transport conduits will be identified by functional, in situ hybridization and localization analysis. Accordingly, distributions of callose at PD and sieve pore, activities of β-l,3-glucanase enzyme and alterations of transport paths during dormancy release are also evaluated to analysis their co-relationship with PsBGs expression. The promoters of PsBGs are cloned, and factors influencing their expression are explored using engineered Arabidopsis. GA response elements in the PsBGs will be identified by deletion analysis and mutation analysis in transgenic Arabidopsis, according to GUS activities. The transcriptional factors involved in regulation PsBGs by GA are then screened by yeast-one-hybridization and identified by EMSA and ChIP. The expression patterns of the identified transcriptional factors are studied, and the regulation mechanism of PsBGs by GA is finally elucidated. These results are of great value to understand the mechanism of dormancy release, the regulation of substance transport during dormancy release, and as an important basement to innovate the forcing culture technology for tree peony.