本研究拟对新疆的疆岳驴、新疆驴、关中驴鲜驴乳中游离脂肪酸进行分离纯化，评价各游离脂肪酸对结核分枝杆菌（Mycobacterium tuberculosis H37Rv）的抑制效果；明确抑菌因子，探讨抑菌因子之间的相互作用；检测抑菌因子对结核分枝杆菌增殖、细胞形态、细胞膜通透性、细胞代谢活力、细胞DNA/RNA合成能力等方面的影响，并分析菌体蛋白质组学等方面，揭示其抑菌机理，以期能为驴奶在临床和食疗方面的应用提供科学依据，从而为结核病的治疗和驴乳的应用开辟新的途径。

The free fatty acids(FFA) of Jiangyue donkey, Guanzhong donkey and Xinjiang donkey were isolated and purified to evaluate its effects on Mycobacterium tuberculosis H37Rv. We will figure out the inhibitory factors of FFA and the interactions between inhibitory factors. To clarify the antibacterial mechanism of inhibitory factors, the morphology of Mycobacterium tuberculosis H37Rv, proliferation, cell membrane permeability, cell metabolism, cell DNA/RNA synthetic ability and cell proteomics were analyzed and detected. Through this study, we can provide a scientific basis for application of donkey milk in the clinical and therapeutic aspects.