胆管从头再生的细胞来源和分子机制研究

批准号:
31970784
项目类别:
面上项目
资助金额:
57.0 万元
负责人:
何建波
依托单位:
学科分类:
组织器官稳态维持与再生修复
结题年份:
2023
批准年份:
2019
项目状态:
已结题
项目参与者:
何建波
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中文摘要
肝脏是人体最重要的消化器官之一;其主要依赖肝脏细胞和胆管细胞来发挥重要生理功能。肝脏细胞在急、慢性肝脏疾病中大量受损后,部分胆管细胞能够进一步转分化为功能性的肝脏细胞并参与再生修复。但到目前为止,对于胆管细胞极度受损后从头再生的细胞来源和分子机制还不清楚。本项目以斑马鱼为模式动物,通过建立斑马鱼胆管极度受损再生模型,致力于鉴定参与胆管从头再生的重要细胞来源并揭示参与再生修复的关键分子机制。本项目包括以下四项研究内容:1)斑马鱼胆管极度损伤修复模型的构建;2)斑马鱼胆管从头再生的主要细胞来源鉴定;3)斑马鱼胆管受损修复过程中的细胞行为和功能机制研究;4)斑马鱼胆管再生的关键分子机制研究。通过发育生物学、分子遗传学等技术深入探讨胆管再生的细胞和分子机制,本项目将进一步揭示肝脏再生的细胞来源和分子机理,对再生医学具有重要意义。
英文摘要
The liver is one of the most important digestive organs in the human body; it plays an important physiological function mainly depends on hepatocytes and cholangiocytes. After the proliferation of hepatocytes is blocked in acute and chronic liver diseases or extreme hepatocytes ablation, the cholangiocytes could be further converted into functional liver cells and participate in liver regeneration. So far, the cell sources and molecular mechanisms involved in biliary duct regeneration are unclear. By using zebrafish as an animal model, this project aims to identify the important cell sources involved in the regeneration of the bile duct and uncover the key molecular mechanisms after extremely biliary duct ablation in zebrafish. This project main content four parts: 1) Construction of biliary duct extreme injury model in zebrafish; 2) Identification of the main cell sources for cholangiocytes regeneration; 3) Uncover the cellular behavior and functional mechanisms during biliary duct regeneration; 4) Exploration the key molecular mechanism involved in bile duct regeneration in zebrafish. Through the developmentalal biology, molecular genetics and other technologies to explore the cell sources and molecular mechanisms of biliary duct regeneration, this project will further discover the cell source and molecular mechanism during the liver regeneration, which is important for regenerative medicine.
期刊论文列表
专著列表
科研奖励列表
会议论文列表
专利列表
Contributions of biliary epithelial cells to hepatocyte homeostasis and regeneration in zebrafish.
胆管上皮细胞对斑马鱼肝细胞稳态和再生的贡献
DOI:10.1016/j.isci.2021.102142
发表时间:2021-03-19
期刊:iScience
影响因子:5.8
作者:Zhang W;Chen J;Ni R;Yang Q;Luo L;He J
通讯作者:He J
Loss of the RNA-binding protein Rbm15 disrupts liver maturation in zebrafish
RNA 结合蛋白 Rbm15 的缺失会破坏斑马鱼的肝脏成熟
DOI:10.1074/jbc.ra120.014080
发表时间:2020-08-14
期刊:JOURNAL OF BIOLOGICAL CHEMISTRY
影响因子:4.8
作者:Hu, Liang;Li, Hongyan;He, Jianbo
通讯作者:He, Jianbo
Combined whole-mount fluorescence in situ hybridization and antibody staining in zebrafish embryos and larvae
斑马鱼胚胎和幼鱼的整体荧光原位杂交和抗体染色相结合
DOI:10.1038/s41596-020-0376-7
发表时间:2020-09-09
期刊:NATURE PROTOCOLS
影响因子:14.8
作者:He, Jianbo;Mo, Dashuang;Luo, Lingfei
通讯作者:Luo, Lingfei
DOI:10.1038/s41536-022-00217-8
发表时间:2022-03-29
期刊:NPJ Regenerative medicine
影响因子:7.2
作者:He J;Zhou Y;Qian C;Wang D;Yang Z;Huang Z;Sun J;Ni R;Yang Q;Chen J;Luo L
通讯作者:Luo L
Tel2 regulates redifferentiation of bipotential progenitor cells via Hhex during zebrafish liver regeneration
Tel2在斑马鱼肝脏再生过程中通过Hhex调节双能祖细胞的再分化
DOI:10.1016/j.celrep.2022.110596
发表时间:2022-04-05
期刊:CELL REPORTS
影响因子:8.8
作者:Zhang,Junren;Zhou,Yang;Luo,Lingfei
通讯作者:Luo,Lingfei
肝脏再生的细胞和分子机制
- 批准号:CSTB2023NSCQ-JQX0004
- 项目类别:省市级项目
- 资助金额:100.0万元
- 批准年份:2023
- 负责人:何建波
- 依托单位:
Reg1作为再生起始因子在肝脏受损修复中的功能和作用机制
- 批准号:31801214
- 项目类别:青年科学基金项目
- 资助金额:27.0万元
- 批准年份:2018
- 负责人:何建波
- 依托单位:
国内基金
海外基金
