从miR-106a/miR-22调控IL-10/STAT-BAFF/BAFFR通路探讨芍药甘草汤对脑缺血再灌注的神经保护机制

芍药甘草汤不仅是止痛第一方，也具有脑保护作用。课题组前期多项临床研究将芍药甘草汤应用于卒中后偏瘫治疗取得较好疗效。前期基础研究也证实了芍甘汤具有抑制脑缺血再灌注(CI/RP)后促炎因子IL-1β，TNF-α等的作用，并上调抑炎因子IL-10的表达。课题组假设芍甘汤通过调控IL-10及其关联的miRNA群而发挥神经保护作用，预实验结果从中筛选了miR-106a和miR-22展开进一步研究。本项目计划：（1）通过临床研究，明确芍药甘草汤对患者血浆中的miR-106a/miR-22表达水平的影响和卒中偏瘫等症状的相关性;（2）通过动物实验和体外实验，明确芍药甘草汤可激活缺血半暗区的miR-106a/miR-22,并通过调控IL-10/STAT-BAFF/BAFFR通路，降低细胞凋亡。本项目的完成将为芍甘汤应用于卒中领域治疗提供实验室依据。

Shaoyao-Gancao decoction is not only the first formula to relieve pain, but also has brain protective effect. A number of our previous clinical studies showed that it has achieved a good effect in the treatment of hemiplegia after stroke. Previous basic studies also confirmed that it can inhibit the pro-inflammatory factors like IL-1β, TNF-α after CI/RP, improve the level of anti-inflammatory factor IL-10. We assumed that peony and licorice decoction exerts a neuroprotective effect by regulating IL-10 and its associated miRNAs, and miR-106a and miR-22 were screened for further study in the preliminary experiment. The project plans to 1) The clinical study was conducted to determine the correlation between the expression of miR-106a/miR-22 in the plasma of patients treated with peony and licorice decoction and the symptoms of stroke and hemiplegia. 2) Animal experiments and in vitro cell experiments were conducted to confirm that peony and licorice decoction can activate miR-106a/miR-22 in the penumbral region and reduce apoptosis by regulating IL-10/STAT-BAFF/BAFFR pathway. The completion of this project will provide a laboratory basis for the application of peony and licorice decoction in the treatment of stroke.