Shaoyao-Gancao Decoction Promoted Microglia M2 Polarization via the IL-13-Mediated JAK2/STAT6 Pathway to Alleviate Cerebral Ischemia-Reperfusion Injury.
Shaoyao-Gancao Decoction Promoted Microglia M2 Polarization via the IL-13-Mediated JAK2/STAT6 Pathway to Alleviate Cerebral Ischemia-Reperfusion Injury.
复制标题
逍遥甘草汤通过IL-13介导的JAK2/STAT6通路促进小胶质细胞M2极化，减轻脑缺血再灌注损伤。
DOI：
10.1155/2022/1707122
复制
发表时间：
2022
影响因子：
4.6
通讯作者：
Yan, Guofeng
中科院分区：
文献类型：
--
作者：
Lu, Juanjuan;Wang, Jie;Yu, Long;Cui, Rong;Zhang, Ying;Ding, Hanqing;Yan, Guofeng
半暗带小胶质细胞在脑缺血再灌注后3 ~ 5天由M2表型转变为M1表型，促进局部炎症和损伤。少药甘草汤（SGD）可导致半影区IL-13的显著上调，从而诱导M2小胶质细胞的极化。因此，有假说认为SGD可能通过激活IL-13诱导小胶质细胞向M2表型极化，从而发挥抗炎和神经保护作用，本研究旨在探讨SGD对小胶质细胞表型转换的影响及其可能机制。脑中动脉闭塞手术（MCAO）大鼠分别给予SGD治疗3、6 d，探讨SGD对脑缺血再灌注损伤（CI/RP）的治疗效果及机制。结果表明，SGD改善神经行为评分，减少细胞凋亡。此外，SGD显著降低了M1小胶质细胞和M1样细胞标志物，但增加了M2小胶质细胞和M2标志物。此外，与MCAO相比，SGD组的IL-13水平和p-JAK2/JAK2和p-STAT6/STAT6的比例更高。总之，我们证实了SGD通过驱动小胶质细胞从M1到M2的表型转换来预防损伤，这可能是通过IL-13及其下游JAK2-STAT6途径实现的。由于本研究未纳入进一步的验证试验，因此有必要进行更多的实验来验证上述结果的可靠性。
Microglia in the penumbra shifted from M2 to M1 phenotype between 3 and 5 days after cerebral ischemia-reperfusion, which promoted local inflammation and injury. Shaoyao-Gancao Decoction (SGD) has been found to result in a significant upregulation of IL-13 in the penumbra, which has been shown to induce polarization of M2 microglia. There was thus a hypothesis that SGD could exert an anti-inflammatory and neuroprotective effect by activating IL-13 to induce microglia polarization towards M2 phenotype, and the purpose of this study was to explore the influence of SGD on microglia phenotype switching and its possible mechanism. Rats who received middle cerebral artery occlusion surgery (MCAO) were treated with SGD for 3 or 6 days, to investigate the therapeutic effect and the underlying mechanism of SGD for cerebral ischemia-reperfusion injury (CI/RP). The results indicated that SGD improved neurobehavioral scores and reduced apoptosis. Furthermore, SGD significantly decreased M1 microglia and M1-like markers, but increased M2 microglia and M2 markers. Moreover, higher levels of IL-13 and ratios of p-JAK2/JAK2 and p-STAT6/STAT6 were found in the SGD group compared to the MCAO. In conclusion, it was verified that SGD prevented injury by driving microglia phenotypic switching from M1 to M2, probably via IL-13 and its downstream JAK2-STAT6 pathway. Given that no further validation tests were included in this study, it is necessary to conduct more experiments to confirm the reliability of the above results.
登录
查看更多内容
影响因子：
6
作者：
Abe N;Nishihara T;Yorozuya T;Tanaka J
通讯作者：
Tanaka J
影响因子：
8.3
作者：
Del Rio-Araiza, Victor H.;Palacios-Arreola, Margarita, I;Morales-Montor, Jorge
通讯作者：
Morales-Montor, Jorge
在中度创伤性脑损伤小鼠模型中，自噬激活通过 IL-13 和 JAK1/STAT1 途径抑制细胞焦亡
影响因子：
5
作者：
Gao, Cheng;Yan, Ya'nan;Tao, Luyang
通讯作者：
Tao, Luyang
影响因子：
2.9
作者：
Liu, Jing;Jin, Dao-Zhong;Zhu, Xing-Zu
通讯作者：
Zhu, Xing-Zu
影响因子：
3.8
作者：
McCormick SM;Heller NM
通讯作者：
Heller NM