模式识别受体识别病原微生物相关分子模式（PAMP）是植物激活先天免疫反应的关键。疫霉菌坏死诱导蛋白来源的nlp20肽段能够被拟南芥受体类蛋白RLP23识别，并激活植物免疫反应。nlp20在多种卵菌、细菌和真菌中都比较保守，是一种具有重要研究意义的PAMP分子。目前对于nlp20诱导的免疫应答及其调控机制尚不清楚。本项目前期鉴定到一个受体激酶蛋白SBP1（SOBIR1-binding protein 1），该蛋白特异性地调控nlp20诱导的活性氧爆发、MAPK激活和防卫基因表达；SBP1与受体RLP23和共受体SOBIR1都存在蛋白互作。本项目拟以此为基础，确定SBP1在nlp20诱导的免疫反应中的功能，明确SBP1与RLP23受体复合体组分的互作关系，解析SBP1参与nlp20免疫信号通路的分子机理，进一步加深对nlp20信号通路的理解，增强对植物与病原微生物互作机制的认知。

Plant pattern-recognition receptors (PRRs) can recognize pathogen-associated molecular patterns (PAMPs) to activate plant innate immunity. Nlp20 is a conserved peptide from the NLP proteins produced by Phytophthora. It is recognized by RLP23, a receptor-like protein (RLP) from Arabidopsis. The nlp20 peptide is widespread within diverse microbial lineages and is considered as an important PAMP. However, the precise regulation mechanism of nlp20-induced immune signaling remains elusive. In this project, we identified the receptor kinase protein SBP1 (SOBIR1-binding protein 1), which interacts with RLP23 receptor and the co-receptor SOBIR1. We observed that the nlp20-induced ROS burst, MAPK activation, and defense gene expression were significantly reduced in the sbp1 mutant. Based on these findings, we propose to further confirm the role of SBP1 in nlp20-triggered immune responses. We plan to analyze the dynamic interaction between SBP1 and the known components of the RLP23 receptor complex. We will elucidate the regulatory mechanism of SBP1 in nlp20-induced signaling and provide a better understanding of plant-microbe interactions.