多环芳烃（PAHs）能够引发多种恶性肿瘤，对其暴露水平进行监测是研究PAHs暴露和预警PAHs中毒的重要前提。检测PAHs暴露标志物——尿中羟基多环芳烃（OH-PAHs）的水平可以综合评价人体对PAHs的暴露程度，然而现有的前处理与检测手段仍存在诸多问题。本项目瞄准以上问题，利用石墨烯作为功能化材料，通过对其结构与性质进行筛选与调控，使其同时具备对OH-PAHs的高效富集能力和灵敏检测性能，在此基础上深入融合固相微萃取（SPME）与表面增强拉曼光谱（SERS）的应用优势，构建基于石墨烯的SPME@SERS一体化分析技术，并研究其萃取和检测OH-PAHs的性能。通过方法学研究，并以高效液相色谱法作为对照，评价其在实际尿样中OH-PAHs检测的应用可行性。该技术的发展与应用将有望突破现有条件下职业卫生监测技术发展的瓶颈，从而为PAHs的生物监测提供一种高效、灵敏的卫生分析化学新方法。

Polycyclic aromatic hydrocarbons (PAHs) can cause cancer under high occupational exposure. Monitoring the level of PAHs will provide scientific evidence for epidemiology research. The PAHs exposure level can be assessed comprehensively by detecting the hydroxy-PAHs (OH-PAHs) in urine. In this project, graphene has been chosen to combine solid phase micro-extraction (SPME) and surface enhanced Raman spectroscopy (SERS), due to its extraordinary properties. Via the structure and property tuning of graphene, we are going to prepare a coating material, which can enrich OH-PAHs and provide sensitive SERS signal of them. Based on this material, the SPME@SERS extracting and detecting integrated technique will be established. The extraction and detection performanceof the integrated technique on OH-PAHs will be investigated. With methodology study and high performance liquid chromatography as control method, the feasibility of SPME@SERS detection of OH-PAHs in real urine samples can be assessed. The development and application of SPME@SERS integrated technique will provide a novel resolution for the fast and sensitive biological monitoring of PAHs in hygienic analytical chemistry, which may break the bottleneck in the development of current occupational monitoring techniques.