黄单胞属病原菌是一类危害严重的植物病原细菌，可引起超过400种单双子叶植物病害，而申嗪霉素可以有效控制黄单胞属病原菌引起的植物病害。申请人前期研究发现细胞色素C合成系统和双精氨酸蛋白转运系统在调控野油菜黄单胞菌对申嗪霉素耐药性过程中发挥着极为重要的作用。本研究中，申请人通过基因编辑技术证明了由细胞色素C合成系统和双精氨酸蛋白转运途径共同组装的电子传递链重要组分细胞色素bc1复合体是调控黄单胞菌属病原菌对申嗪霉素及其同系物耐药性的关键酶体。本研究后续拟以野油菜黄单胞菌为研究对象，通过探究电子传递链不同组分（NADH脱氢酶、琥珀酸脱氢酶、细胞色素c蛋白）在调控对申嗪霉素耐药性过程中的作用以及细胞色素bc1复合体缺陷对电子传递链不同组分功能的影响，明确细胞色素bc1复合体调控黄单胞菌对申嗪霉素耐药性的分子机制，为研究如何提高申嗪霉素的作用效果奠定基础。

Xanthomonas spp., a class of bacterial plant pathogens, can infect over 400 kinds of plants causing severe yield and quality loss worldwide, and Phenazine-1-carboxylic acid (PCA) can effectively control the plant diseases caused by Xanthomonas spp.. The cytochrome c maturation (CCM) system and twin-arginine translocation (TAT) pathway were found to play an important role in regulating the tolerance of Xcc to PCA. In this study, it is demonstrated the cytochrome bc1 complex, which is matured by CCM system and TAT pathway together, regulates the PCA tolerance more directly in Xanthomonas spp.. Represented by Xcc, we are going to clarify the regulation mechanism of the cytochrome bc1 complex to PCA tolerance by exploring the influence of cytochrome bc1 complex to other components of the electron transport chain, and by researching the relationship between the PCA tolerance and the function of different electron transport chain components.