功能性消化不良（FD）是临床上最常见的内科疾病之一，也是中医药治疗的优势病种。本病发病机制尚不完全明确，最新研究发现十二指肠功能异常是FD重要发病机制，而HCO3-的分泌可有效恢复十二指肠正常功能。本课题拟通过动物实验和体外细胞实验，从中药“健脾理气方”通过提高5-HT和PGE2组织含量激活十二指肠上皮细胞内的PKA和PKC信号，作用于IRBIT蛋白协同调控Na+/ HCO3-共转体（NBC1）促进HCO3-分泌的角度，探究其可能的作用靶点和信号介导途径。以期明确健脾理气方治疗FD的分子机制，并从分子生物学水平为中药在FD治疗中的优势提供理论依据。

Functional dyspepsia (FD) is one of the complex gastrointestinal diseases with an increasing incidence, but the pathological process and mechanisms of FD remains unclear. Recently, a line of evidence implicates duodenal dysfunction as a major cause of FD, and stimulation of HCO3- can ameliorate duodenal health. Chinese medicine, Jianpiliqi formula, has demonstrated dramatic therapeutic effects in the treatment of FD. The major goal of the current proposal is to delineate the molecular mechanisms by which Jianpiliqi formula improves symptoms of FD. We will test the hypothesis that Jianpiliqi formula could promote the secretion of 5-HT and PGE2 in the duodenum eliciting PKA and PKC activation, which in turn synergistically activates NBC1 membrane localization and HCO3-secretion via IRBIT. We anticipate the current work will promote our understanding of the molecular mechanisms of FD treatment by Jianpiliqi fomula, and provide molecular basis for the advantages of using Chinese medicine in treating FD.