免疫反应是组织再生修复的重要阶段，主动和精确调控免疫反应是组织再生材料研究的前沿课题。本项目针对组织再生过程免疫调控难题，以巨噬细胞表型及其动态转变对组织再生的重要作用为启示，结合材料表面图案对巨噬细胞表型的调控规律，构建一种具有热、光响应的动态图案化基底，实现巨噬细胞表型动态调控，探索表型动态转变对组织修复相关细胞行为的影响。合成可在生理温度范围变形的形状记忆聚己内酯（PCL），结合金纳米棒的优异光热转化效应和近红外光的高度空间选择性，构建近红外光响应的金纳米棒/形状记忆PCL动态图案化基底。以光为开关，实现动态图案化基底对巨噬细胞表型和组织修复相关细胞行为的调控。阐明基底表面图案特征及其动态转变对巨噬细胞表型的调控规律，揭示基底动态表界面特征—巨噬细胞表型—组织修复相关细胞行为间的相互影响机制。为深入理解材料-细胞相互作用，发展具有主动免疫调控性能的新型组织再生材料提供理论依据。

Immune response is a necessary and important stage of tissue repair and regeneration. Proactive and precise regulations of immune response are at the leading edge in the design of tissue regeneration materials. Macrophages have been regarded as a primary target in regulating immune response because of their critical roles in regulating multiple phases of tissue repair through their unique ability to rapidly shift phenotypes. Aiming at the problem of immune regulation in tissue regeneration, the study of designing a thermo- or light- responsive patterned surface with the property to topographical control the phenotypes of macrophage dynamically is proposed. And further explore the influence of transformed phenotypes of macrophage on the tissue repair-related cells. The immune regulation material is designed based on the explored relationship between material surface patterns and macrophage phenotypes. The shape memory poly-caprolactone (PCL) that functions near physiological temperature was synthesized, and combined with gold nano-rods which own excellent photo-thermal conversion effect to fabricate a near-infrared-responsive patterned PCL substrate, endowing with the properties of topographical transformation and macrophage phenotypes regulation. The macrophage phenotypes and the fate of tissue repair- related cells can be dynamically regulated by the switch of high spatial selective light. The influences of the surface pattern on the macrophage phenotypes will be elucidated, and the interactions among the dynamic surface pattern, the macrophage phenotypes and the behaviors of tissue repair-related cells will be revealed, providing theoretical support for the further understanding of material-cell interactions and the development of tissue regeneration materials with proactive immune regulation properties.