抗生素抗性基因是一种新型的环境污染物，其污染特征和环境分布是目前环境科学研究的热点之一，相关研究主要集中在污水处理厂、畜禽养殖场及其受纳环境，而对水产养殖环境，特别是水产品中抗生素抗性基因的相关研究非常有限。本项目拟应用实时荧光定量PCR技术，分析对虾养殖过程中水体、沉积物、饲料、微生态制剂等环境介质和虾体中典型抗生素抗性基因的污染类型、数量变化，阐明对虾养殖过程中抗生素抗性基因在养殖环境多介质中的污染特征；综合运用多种统计学方法分析环境因子与抗生素抗性基因在水体和沉积物中污染变化的关系，揭示其对抗生素抗性基因污染变化的影响；应用高通量测序技术分析对虾（虾苗和成虾）肠道与环境介质的微生物群落结构特征，揭示对虾养殖过程中抗生素抗性基因在养殖环境中的传播机制。本项目为保证我国水产品安全提供数据支持，为进一步完善和提升我国的水产健康养殖技术水平提供科学依据。

Antibiotic resistance genes (ARGs) are emerging environmental contaminants. The contamination characteristics and distribution of ARGs are currently hot topics in the environmental science. Related research mainly focused on the sewage treatment plants, livestock farms and rivers. The research of ARGs in the aquaculture is limited, especially in the aquatic products. The objective of this project is to (i) illuminate the contamination characteristics of ARGs of multimedium in the shrimp breeding, by analyzing the types and levels of ARGs in the water, sediment, feeds and shrimp body in the process of shrimp breeding with the fluorescence quantitative PCR technology, (ii) reveal its influence on the contamination levels change of ARGs in the water and sediment with various statistical methods, by analyzing the environmental factor, (iii) and reveal the dissemination of ARGs in the aquaculture environment, by analyzing the microbial community structure information of shrimp (seed shrimp and adult shrimp) intestinal tract and environmental mediums with high-throughput sequencing technology. This project provides data support and scientific basis for ensuring the safety of aquatic products, and for further improving and promoting healthy aquatic breeding technology in China.