氢能源的发展利用是实现“碳达峰”“碳中和”的关键。绿藻光生物学制氢由于净零碳排放、具有大规模应用前景而愈发受到关注。目前，绿藻光生物学制氢的实际推广受限于高成本厌氧环境的构建。最新研究发现，游离的绿藻聚集后，可以自发在聚集体内部形成厌氧环境而进行光生物学制氢，具有降低制氢成本的潜力。然而，绿藻聚集体自身耗氧能力较弱，形成高效制氢的厌氧环境需要较长的预孵育时间，导致了整体制氢速率不高。本项目针对厌氧环境构建这一瓶颈问题，提出将自身耗氧与外部除氧两种手段相结合，以实现低成本地构建出能够快速高效制氢的厌氧环境。首先，通过快速制备绿藻聚集体，以及时获得自身耗氧能力，然后，加入少量能够高效除氧的化学-酶级联反应组分，以及时去除聚集体外部绿藻产生的氧气，最后，绿藻聚集体整体快速地进入厌氧环境，从而实现在低成本条件下绿藻快速高效光生物学制氢。本项目的实施对绿藻光生物学制氢的应用发展具有重要意义。

The development and utilization of hydrogen energy is the key to reaching carbon dioxide emissions peak and achieving carbon neutrality. The green algae-based photobiological hydrogen production has attracted more and more attention due to its net-zero carbon emission and the advantages of large-scale application. The current approaches used to create the anaerobic environment required for green algae-based photobiological hydrogen production are expensive, which limits their actual promotion. New research indicates that after free green algae aggregate, they can spontaneously form an anaerobic environment inside the aggregate for photobiological hydrogen production, which has the potential to reduce the cost of hydrogen production. However, the ability of the aggregates to consume oxygen is poor, leading to a long pre-incubation time to form an anaerobic environment for efficient hydrogen production, resulting in a low overall hydrogen production rate. Aiming at the bottleneck problem of anaerobic environment construction, this project proposes to achieve low-cost construction of an anaerobic environment for green algae producing hydrogen quickly and efficiently by combining the ability of oxygen consumption from the green algae aggregates themselves and external oxygen removal method. First, through the rapid preparation of green algae aggregates to obtain their oxygen consumption ability in a timely. Then, a small number of chemoenzymatic cascade components that can efficiently remove oxygen are added to remove the oxygen produced by the green algae outside the aggregates in a timely. Finally, the aggregates quickly enter the anaerobic environment as a whole, thereby realizing fast and efficient green algae-based photobiological hydrogen production under low-cost conditions. The implementation of this project is of great significance to the application and development of green algae-based photobiological hydrogen production.