基于肽核酸入侵的微电极阵列高灵敏双链DNA直接测定研究

用电学方法在纳米器件上检测核酸分子是一种新兴的检测技术，通常仍采用Watson-Crick的碱基配对原则对单链DNA的特异性识别；但是如何在微电极阵列上直接检测生物样品中的双链DNA分子，依然是一个未解决的科学问题。本项目拟通过开展基于叉指阵列微电极纳米器件表面的肽核酸(PNA)探针入侵双链DNA分子的超灵敏DNA直接检测研究，致力于探讨叉指阵列微电极器件微纳界面的可控自组装体系及PNA与双链DNA (dsDNA)的相互作用，及其对核酸分子检测和分子诊断的影响；设计具有分子识别功能导向的自组装新体系和新技术，研究叉指阵列微电极器件构筑的生物传感器对于目标核酸及单核苷酸多态性的检测性能，从理论上来探索微阵列电极上直接检测生物样品的双链DNA分子的机理和方法，实现对双链DNA分子的高选择性、超灵敏直接检测。这对于建立基于纳米生物技术的新型电检测生物传感系统具有重大的意义。

Detection of nucleic acids on nano device with electrical methods is an emerging detection technology, in which Watson-Crick base pairing rules are generally adopted to specifically identify single-strand DNA. But how to directly detect dsDNA on microelectrode array has been an unsolved scientific problem. In this project, we will develope a direct and ultra-sensitive electronic DNA biosensor based on the invasion of peptide nucleic acid (PNA) probe on interdigitated array microelectrode into target double-stranded DNA (dsDNA). For this purpose, attentions would be paid on exploring the controllable self-assembly system on the surface of interdigitated array microelectrode; investigating the interaction of PNA with dsDNA; studying the influence on nucleic acids detection and molecular diagnosis; designing new self-assembly system and technology with molecular recognition function; researching on the detection performance of the biosensor fabricated by interdigitated array microelectrode toward target nucleic acids and single nucleotide polymorphisms. This biosensor is for the highly selective, sensitive and direct detection of dsDNA molecules. It is of great significance for building new electric biosensing system based on nano biotechnology and exploring its application in highly sensitive detection of cancer and other serious diseases.