生物电化学系统（BES）阳极能降解有机物的同时回收重金属，且不需额外投加物，为含重金属有机废水处理提供了新途径。但在有机物、重金属离子和阳极电极共存下，重金属离子与电活性生物膜的互作机理尚不清楚。本项目以两种重金属离子Cu2+和Cd2+为研究对象，构建BES阳极（硫还原地杆菌）处理含重金属有机废水体系，通过能谱和成像分析重金属离子在电活性生物膜-溶液界面的形态转化和传输过程，结合物质平衡及库伦效率计算，揭示重金属离子与阳极间的电子竞争机制，阐明重金属在BES阳极的界面迁移与转化机理。采用共聚焦荧光显微分析电活性生物膜的生物活性，结合循环伏安、电化学阻抗及胞外聚合物中传导性组分细胞色素c的定量分析，描述其在重金属离子存在下的变化过程，以明确重金属离子对阳极电活性生物膜生理效应与电化学特性的影响规律，揭示重金属-电活性生物膜的互作机理，为BES阳极实现高效的污染物转化并同时回收重金属提供依据。

The anode of bioelectrochemical systems (BES) have shown promising performance in terms of achieving heavy metal ions removal with simultaneous organics removal, even with electricity generation. However, the interactions between heavy metal ions and anodic electroactive biofilms have not been studied. In this study, two heavy metals (Cu2+ and Cd2+) will be selected to investigate their impacts on the anode(G. sulfurreducen) of BES. The transport and transfer mechanism of heavy metals ions will be studied using energy dispersive spectroscopy and X-ray photoelectron spectroscopy. In addition, the physiological and electrochemical effects (microbial morphology, biofilms structure and distribution, biofilms conductivity, nanowires for electron transfer, extracellular polymers c) of typical heavy metal ions on electroactive biofilms will be considered using microbial and electrochemical methods, such as cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Finally, the interactions between heavy metal ions and anodic electroactive biofilms and could be proposed. This study will provide a scientific basis for practical application of BES, especially in the wastewater with heavy metal ions and organic matters.