大白菜耐热性LncRNAs的调控网络分析及功能研究

大白菜（Brassica rapa ssp. pekinensis）起源于中国，是重要的世界性蔬菜和园艺作物。高温严重阻碍了大白菜的周年生产，影响了其产量和品质。同时，LncRNAs研究成为近年来植物研究领域的一个新热点，然而在大白菜耐热研究中LncRNAs的调控机制还未见报道。本项目在已完成的大白菜LncRNAs测序的基础上，在全基因组范围内鉴定高温胁迫相关LncRNAs，并构建其与逆境相关转录因子的共表达调控网络。并进一步对高温胁迫关键LncRNAs及靶基因进行全长克隆、时空表达分析、亚细胞定位及转基因功能验证。通过系统深入的研究，全面揭示LncRNAs在大白菜耐热中的调控机理。有助于加深人们对芸薹属及其近缘物种耐热性的认识，为培育和创制耐热优良新品种奠定基础。该研究不仅具有重要的理论价值，而且有着突出的经济价值。同时，建立的分析流程将为其它植物LncRNAs的研究提供重要支持平台。

Chinese Cabbage (Brassica rapa ssp. pekinensis) originated in China, is an important worldwide vegetable and horticultural crops. High temperature severely hampered annual production of cabbage, affecting its yield and quality. Meanwhile, LncRNAs has become a new hot spot in the field of plant research in recent years. However, the regulation mechanism of LncRNAs has not been reported for the study of heat tolerance in Chinese cabbage. Based on the completion of the LncRNAs sequencing in Chinese cabbage, this project try to identify the high temperature stress related LncRNAs in the whole genome. Then, we construct the co-expression regulatory network of these LncRNAs and stress related transcription factors. Furthermore, the full length cloning, spatial and temporal expression analysis, subcellular localization and functional verification of key LncRNAs and their target genes related to high temperature stress were studied. Through systematic and in-depth study, we try to reveal the regulation mechanism of LncRNAs in the heat tolerance of Chinese cabbage. This project helps to deepen the understanding of heat resistance for Brassica species and related species, and laid the foundation for breeding and creating new varieties of heat resistance. This research not only has great scientific significance value, but also has prominent economic value. At the same time, the research streamline constructed will be an essential platform for the study of LncRNAs in other plant.