昆虫肠道细菌因其与昆虫间共生关系及其对昆虫的生理调节功能而备受关注，国内外对一些仅以纤维素为主要成份植物组织为食的昆虫肠道细菌多样性及食性适应机理等研究方面已取得了重要成果，但对能以纤维素和淀粉为营养成分植物组织为食的昆虫肠道细菌多样性及食性适应机理研究还未见报道。马铃薯块茎蛾是一种既能取食以纤维素为主要成分的马铃薯叶片、又能取食以淀粉为主要成分的马铃薯块茎的国际检疫性害虫，其肠道细菌多样性及食性的适应机制还不清楚。本项目拟以马铃薯块茎蛾为材料，采用16S rDNA扩增子测序法，研究继代取食马铃薯叶片、薯块、叶片和薯块混合寄主的马铃薯块茎蛾幼虫肠道细菌种类组成及优势种类；再通过高深度宏基因组测序及宏转录组技术研究肠道细菌对纤维素和淀粉的降解功能，探明马铃薯块茎蛾对富含纤维素的叶片和富含淀粉的薯块的适应机制。该研究结果将为马铃薯块茎蛾肠道微生物多样性研究及害虫防治提供新的思路和科学依据。

Study on the insect insect gut bacterials has been concerned just because of the interaction between the insect gut bacterials and the meditate functions for insects, great achievements have been get on the gut microrganisms diversity and the mechanism for the feeding habitat adapty for theose insects feeding soly on the cellulose plants, but no resports on the gut bacterials diversity and the mechanism for the feeding habitat adapty for insects feeding both on the cellulose and amylum plants. The Potato Tuber Moth, Phthorimaea operculella Zeller, is an important pest insect of potato, it can feeding on leaf and tuber of potato, and it is a quarantine insect, the gut bacterials diversity and the mechanism for the feeding habitat adapty for potato tuber moth is unclear. This study will study the diversity and the predominant species of gut bacterials from the gut of those potato tuber moth larvae after the successive fed with potato leaf, potato tuber and leaf tuber combined feeds using the 16S rDNAaequencing. The catabolic function of the gut bacterials on the cellulose and amylum using the high depth metagenomics and metatranscriptomics techniques, in order to make clear on the feeding habitat adaption mechanisms for potato tuber moth. The resutls of this study will provide new idear for the study on the diversity of gut microorganisms and scientific bases for the insect control on potato tuber moth.