已有数据表明调控花药发育的多数信号分子在不同物种均有同源基因并且功能类似，暗示开花植物调控花药发育机制的保守性，说明在模式植物中进行花药发育机制研究具有广谱意义。胞外多肽激素CLE家族是在植物中广泛分布的信号分子家族，在很多重要生长发育过程中发挥重要功能。本课题前期研究发现，CLE19在花药绒毡层和生殖细胞中高表达，其功能缺失造成植株雄性育性降低，表明CLE19在花药发育过程中发挥重要作用。因此，本项目拟通过筛选CLE19的膜表面受体，以及对CLE19的功能缺失和过表达突变体的转录组、蛋白及磷酸化组分析，结合深入的分子遗传、生物化学和细胞生物学研究，揭示CLE19激活膜表面受体进而激活下游信号转导通路的分子机制。以期深入认识胞外配基-受体激酶复合物介导的细胞-细胞间通讯在生殖发育过程中的功能，同时为农业生产上对作物进行遗传改良、或者借助胞外小分子多肽诱导改变作物育性和产量提供重要理论指导。

Previous data showed that most known anther developmental-required genes have homologues that exhibited similar functions in different species, suggesting the conservation of the mechanisms that regulating the anther development and the broad spectrum of meanings to study these mechanisms in model plants. The CLE family is an important peptide hormone family that plays important role in many plant growth and developmental processes. Previous study in our group showed that the CLE19 is highly expressed in tapetum and reproductive cells. Both loss–of-function and gain-of-function mutants of CLE19 exhibited obviously defective male fertility, suggesting the CLE19 is required for anther development. In this study, we plan to screen the membrane-localized receptor of CLE19, and analyze the transcriptome and proteome of the CLE19 mutants, and together with further genetic, biochemistry, cell biology studies to demonstrate the signaling pathways and molecular mechanisms that how CLE19 regulates the normal anther development. Thus, this project will provide strong academic evidences and theoretical supports on manipulation of crop to increase the fertility and the production.