Global Quantitative Proteomics Studies Revealed Tissue-Preferential Expression and Phosphorylation of Regulatory Proteins in Arabidopsis.
Global Quantitative Proteomics Studies Revealed Tissue-Preferential Expression and Phosphorylation of Regulatory Proteins in Arabidopsis.
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全球定量蛋白质组学研究揭示了拟南芥中调节蛋白的组织优先表达和磷酸化。
DOI：
10.3390/ijms21176116
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发表时间：
2020-08-25
影响因子：
5.6
通讯作者：
Chang F
中科院分区：
文献类型：
--
作者：
Lu J;Fu Y;Li M;Wang S;Wang J;Yang Q;Ye J;Zhang X;Ma H;Chang F
植物的器官发生发生在生命周期的所有阶段。尽管以前的研究已经在RNA水平上发现了许多对营养或生殖发育重要的基因，但关于翻译和翻译后水平的全球信息仍然有限。本研究利用定量蛋白质组学和磷酸化蛋白质组学方法对拟南芥的6个发育阶段/器官进行了分析，鉴定出2187个非冗余蛋白和1194个磷酸化蛋白。与在茎生叶中观察到的表达相比，1445、1644和1377蛋白在第1-9期花、第10-12期花和开放花中的表达分别发生了1.5倍以上的变化。其中，新发现了294个磷酸蛋白，472个磷酸化位点，其中275个具有差异表达模式，为功能研究提供了分子标记和可能的候选。在花药(15)、小母细胞(4)或花粉(15)中优先表达的基因编码的蛋白质在生殖器官中丰富，两个花药优先表达的蛋白质acos5和mee48的突变体显示出明显的雄性可育性降低，花粉外壁组织异常。此外，在生殖阶段(1149个)比营养器官(995个)鉴定出更多的磷酸化蛋白。Western印迹分析证实GRP17、CDC2/CDKA.1和ATSK11基因具有花器官特异性磷酸化。此外，在生殖组织中，CDPK6和MAPK6及其相互作用蛋白的磷酸化水平升高。总体而言，我们的研究获得了关于六个阶段/器官的蛋白质表达和磷酸化的广泛数据，并为未来研究植物发育的调控机制提供了重要的资源。
Organogenesis in plants occurs across all stages of the life cycle. Although previous studies have identified many genes as important for either vegetative or reproductive development at the RNA level, global information on translational and post-translational levels remains limited. In this study, six Arabidopsis stages/organs were analyzed using quantitative proteomics and phosphoproteomics, identifying 2187 non-redundant proteins and evidence for 1194 phosphoproteins. Compared to the expression observed in cauline leaves, the expression of 1445, 1644, and 1377 proteins showed greater than 1.5-fold alterations in stage 1–9 flowers, stage 10–12 flowers, and open flowers, respectively. Among these, 294 phosphoproteins with 472 phosphorylation sites were newly uncovered, including 275 phosphoproteins showing differential expression patterns, providing molecular markers and possible candidates for functional studies. Proteins encoded by genes preferentially expressed in anther (15), meiocyte (4), or pollen (15) were enriched in reproductive organs, and mutants of two anther-preferentially expressed proteins, acos5 and mee48, showed obviously reduced male fertility with abnormally organized pollen exine. In addition, more phosphorylated proteins were identified in reproductive stages (1149) than in the vegetative organs (995). The floral organ-preferential phosphorylation of GRP17, CDC2/CDKA.1, and ATSK11 was confirmed with western blot analysis. Moreover, phosphorylation levels of CDPK6 and MAPK6 and their interacting proteins were elevated in reproductive tissues. Overall, our study yielded extensive data on protein expression and phosphorylation at six stages/organs and provides an important resource for future studies investigating the regulatory mechanisms governing plant development.
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影响因子：
14.9
作者：
Durek P;Schmidt R;Heazlewood JL;Jones A;MacLean D;Nagel A;Kersten B;Schulze WX
通讯作者：
Schulze WX
DOI：
10.1111/tpj.12170
发表时间：
2013-06
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Ali MA;Plattner S;Radakovic Z;Wieczorek K;Elashry A;Grundler FM;Ammelburg M;Siddique S;Bohlmann H
通讯作者：
Bohlmann H
影响因子：
3.7
作者：
Buxdorf K;Yaffe H;Barda O;Levy M
通讯作者：
Levy M
影响因子：
7.2
作者：
DeYoung, BJ;Bickle, KL;Clark, SE
通讯作者：
Clark, SE
影响因子：
11.6
作者：
Colcombet, J;Boisson-Dernier, A;Schroeder, JI
通讯作者：
Schroeder, JI